Open Access

IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway

  • Authors:
    • Qianya Su
    • Fei Wang
    • Zhengbang Dong
    • Mei Chen
    • Rong Cao
  • View Affiliations

  • Published online on: August 3, 2020     https://doi.org/10.3892/mmr.2020.11403
  • Pages: 3111-3116
  • Copyright: © Su et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the role of janus kinase (JAK)1/STAT1 in interferon (IFN)‑γ‑induced apoptosis in human melanocytes. Following IFN‑γ treatment, the viability of human melanocytes were analyzed using a Cell Counting Kit‑8 assay and the apoptotic rate was determined using flow cytometry. Western blotting was also performed to analyze the phosphorylation levels of JAK1, JAK2 and the transcriptional factor STAT1, as well as the expression levels of Bcl‑2, Bax, Bcl‑2 homologous antagonist killer (Bak) and cleaved caspase‑3. Finally, following the pretreatment with the STAT1 inhibitor fludarabine, human melanocytes were treated with IFN‑γ and flow cytometry was used to detect the apoptotic rate. The results revealed that IFN‑γ reduced the proliferation and induced the apoptosis of human melanocytes. In addition, IFN‑γ treatment led to decreased expression levels of Bcl‑2 and increased expression levels of Bax, Bak and cleaved caspase‑3, alongside the activation of the JAK1/STAT1 signaling pathway. Conversely, the pretreatment with the STAT1 inhibitor fludarabine decreased the apoptotic rate of human melanocytes following IFN‑γ induction. In conclusion, the findings of the present study suggested that IFN‑γ may induce the apoptosis of human melanocytes by activating the JAK1/STAT1 signaling pathway, alongside increasing the expression levels of Bax, Bak and cleaved caspase‑3, and decreasing the expression levels of Bcl‑2.
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October-2020
Volume 22 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Su Q, Wang F, Dong Z, Chen M and Cao R: IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway. Mol Med Rep 22: 3111-3116, 2020.
APA
Su, Q., Wang, F., Dong, Z., Chen, M., & Cao, R. (2020). IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway. Molecular Medicine Reports, 22, 3111-3116. https://doi.org/10.3892/mmr.2020.11403
MLA
Su, Q., Wang, F., Dong, Z., Chen, M., Cao, R."IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway". Molecular Medicine Reports 22.4 (2020): 3111-3116.
Chicago
Su, Q., Wang, F., Dong, Z., Chen, M., Cao, R."IFN‑γ induces apoptosis in human melanocytes by activating the JAK1/STAT1 signaling pathway". Molecular Medicine Reports 22, no. 4 (2020): 3111-3116. https://doi.org/10.3892/mmr.2020.11403