Open Access

Hypoxia in utero increases the risk of pulmonary hypertension in rat offspring and is associated with vasopressin type‑2 receptor upregulation

  • Authors:
    • Hao Ding
    • Yuchuan Luo
    • Ke Hu
    • Huaying Huang
    • Pei Liu
    • Mengqing Xiong
    • Li Zhu
    • Jing Yi
    • Ying Xu
  • View Affiliations

  • Published online on: September 23, 2020     https://doi.org/10.3892/mmr.2020.11533
  • Pages: 4173-4182
  • Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Pulmonary hypertension (PH) in newborns and adults is a disease that can lead to right heart failure and result in a shorter lifespan. PH was induced by maintaining pregnant rats in a hypoxic chamber for 4 h twice a day, from days 7‑21 of pregnancy. Hypoxia was confirmed by a decrease in the partial pressure of oxygen (PaO2) and the oxygen saturation (SaO2) of arterial blood in the aorta. The body weight of newborns from hypoxic rats was ~20% decreased compared with the control newborns of normoxic rats. The vascular wall thickness/vascular diameter values of hypoxia treated pubs were increased compared with that of control newborns 7 days after birth; however, it decreased to similar levels than in the control group after 3 months, and then further decreased to significantly lower levels than in the control group at 6 months after birth. At birth, the lung tissues of newborns from hypoxic rats exhibited an increase in the levels of mRNA and proteins associated with PH such as HIF‑1α, HIF‑2α, V2R, TGF‑β, TNF‑α, Ang‑2 and α‑SMA. At 3 and 6 months after birth, the levels of both V2R mRNA and protein in offspring from hypoxic rats were at least 2‑fold higher, whereas the expression of all other factors decreased compared with the control offspring. By contrast, HIF‑2α and Ang‑2 expression levels were significantly increased in the 6‑month‑old control offspring from normoxic rats. V2R overexpression in pups induced by hypoxia in maternal rats was sustained until their adulthood. V2R may be a marker for detecting PH.
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November-2020
Volume 22 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ding H, Luo Y, Hu K, Huang H, Liu P, Xiong M, Zhu L, Yi J and Xu Y: Hypoxia in utero increases the risk of pulmonary hypertension in rat offspring and is associated with vasopressin type‑2 receptor upregulation. Mol Med Rep 22: 4173-4182, 2020.
APA
Ding, H., Luo, Y., Hu, K., Huang, H., Liu, P., Xiong, M. ... Xu, Y. (2020). Hypoxia in utero increases the risk of pulmonary hypertension in rat offspring and is associated with vasopressin type‑2 receptor upregulation. Molecular Medicine Reports, 22, 4173-4182. https://doi.org/10.3892/mmr.2020.11533
MLA
Ding, H., Luo, Y., Hu, K., Huang, H., Liu, P., Xiong, M., Zhu, L., Yi, J., Xu, Y."Hypoxia in utero increases the risk of pulmonary hypertension in rat offspring and is associated with vasopressin type‑2 receptor upregulation". Molecular Medicine Reports 22.5 (2020): 4173-4182.
Chicago
Ding, H., Luo, Y., Hu, K., Huang, H., Liu, P., Xiong, M., Zhu, L., Yi, J., Xu, Y."Hypoxia in utero increases the risk of pulmonary hypertension in rat offspring and is associated with vasopressin type‑2 receptor upregulation". Molecular Medicine Reports 22, no. 5 (2020): 4173-4182. https://doi.org/10.3892/mmr.2020.11533