MicroRNA‑491‑5p inhibits trophoblast cell migration and invasion through targeting matrix metalloproteinase‑9 in preeclampsia

Liu,Enling; Zhou,Yuxiu; Li,Jun; Zhang,Donghong

doi:10.3892/mmr.2020.11604

MicroRNA‑491‑5p inhibits trophoblast cell migration and invasion through targeting matrix metalloproteinase‑9 in preeclampsia

Authors:
- Enling Liu
- Yuxiu Zhou
- Jun Li
- Donghong Zhang
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Affiliations: Department of Obstetrics and Gynecology, Tangshan Gongren Hospital, Hebei Medical University, Tangshan, Hebei 063000, P.R. China, Department of Immunity, Tangshan Gongren Hospital, Hebei Medical University, Tangshan, Hebei 063000, P.R. China
Published online on: October 14, 2020 https://doi.org/10.3892/mmr.2020.11604
Pages: 5033-5040
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Insufficient invasion of trophoblasts is correlated with the development of preeclampsia (PE). MicroRNA (miR)‑491‑5p has been reported to be implicated in human cancer cell invasion; however, whether miR‑491‑5p is involved in the development of PE remains largely unclear. The aim of the present study was to investigate the role of miR‑491‑5p in trophoblastic invasion in vitro and to determine its underlying mechanism of action. The expression levels of miR‑491‑5p were validated using reverse transcription‑quantitative PCR. The effects of miR‑491‑5p on trophoblast cell invasion were evaluated in vitro. Then, the association between miR‑491‑5p and its downstream target was investigated in both cell lines and clinical specimens. miR‑491‑5p expression levels were observed to be significantly increased in the placental tissues from patients with PE. The invasive capacity of HTR‑8/SVneo trophoblast cells was suppressed following the upregulation of miR‑491‑5p and increased following the inhibition of miR‑491‑5p. Matrix metalloproteinase‑9 (MMP‑9), a well‑known regulator of trophoblast cell invasion, was discovered to be a direct target of miR‑491‑5p in HTR‑8/SVneo trophoblast cells. Moreover, miR‑491‑5p expression levels were found to be inversely correlated with MMP‑9 expression levels in placental tissues from patients with PE. The overexpression of MMP‑9 partly attenuated the inhibitory effects of miR‑491‑5p on HTR‑8/SVneo trophoblast cells invasion. Collectively, these findings suggested that the aberrant expression of miR‑491‑5p may contribute to PE through suppressing trophoblast invasion, thus highlighting the novel roles of miR‑491‑5p in the molecular pathogenesis of PE. The present study also showed that the miR‑491‑5p/MMP‑9 axis may be an effective biomarker or a viable drug target for therapeutic intervention in PE.

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