Tripartite motif‑containing 14 regulates cell proliferation and apoptosis in cervical cancer via the Akt signaling pathway Retraction in /10.3892/mmr.2023.13100

Diao,Wenjing; Zhu,Caiying; Guo,Qisang; Cao,Yuankui; Song,Yu; Feng,Hua; Li,Jun; Xue,Xiaohong; Lu,Pei

doi:10.3892/mmr.2020.11634

Tripartite motif‑containing 14 regulates cell proliferation and apoptosis in cervical cancer via the Akt signaling pathway

Retraction in: /10.3892/mmr.2023.13100

Authors:
- Wenjing Diao
- Caiying Zhu
- Qisang Guo
- Yuankui Cao
- Yu Song
- Hua Feng
- Jun Li
- Xiaohong Xue
- Pei Lu
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Affiliations: Medical Center of Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China, Department of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China, Department of Clinical Laboratory, Shanghai No. 8 People's Hospital, Shanghai 200235, P.R. China
Published online on: October 26, 2020 https://doi.org/10.3892/mmr.2020.11634
Pages: 5145-5154
Copyright: © Diao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tripartite motif‑containing (TRIM) 14 is a protein of the TRIM family. Studies have indicated that TRIM14 may be used as an oncogene in tumor cells, such as osteosarcoma, non‑small cell lung cancer and breast cancer through different pathways. However, the functions of TRIM14 in cervical cancer cells remain unclear. Therefore, this study aimed to investigate the functions of TRIM14 in cervical cancer cells and its underlying mechanism. Caski cells stably expressing TRIM14 and SiHa, and HeLa cells stably expressing TRIM14 short hairpin RNA were constructed by lentivirus‑mediated overexpression or knockdown systems. The effects of TRIM14 on proliferation and apoptosis of cervical cancer cells were detected by Cell Counting Kit‑8 (CCK‑8) assay and flow cytometry, respectively. In addition, reverse transcription‑quantitative (RT‑q) PCR and western blotting were used to investigate the expression levels of TRIM14 and of signaling pathway marker protein including P21, caspase‑3, cleaved caspase‑3, Akt and phosphorylated Akt. The results of RT‑qPCR and western blotting revealed that TRIM14 was highly expressed in human cervical cancer tissues and cell lines compared with adjacent normal tissues and normal cervical epithelial cells. TRIM14 also regulated cell proliferation and apoptosis of human SiHa, HeLa and Caski cervical cancer cell lines through the Akt signaling pathway. Additionally, TRIM14 protein levels were related to the clinical and pathological features of cervical cancer. CCK‑8 assay and flow cytometry demonstrated that TRIM14 expression could promote cervical cancer cell proliferation and autophagy suppression. Taken together, TRIM14‑induced cell proliferation and apoptosis inhibition may by evoked by the activation of the Akt pathway. This study demonstrated the role of TRIM14 in cervical cancer, and reveals its mechanism of action as a potential therapeutic target for cervical cancer.

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