Scutellarin regulates osteoarthritis <em>in vitro</em> by inhibiting the PI3K/AKT/mTOR signaling pathway

Ju,Shao-Hua; Tan,Li-Rong; Liu,Pan-Wang; Tan,You-Li; Zhang,Yuan-Ting; Li,Xiao-Hong; Wang,Ming-Jian; He,Ben-Xiang

doi:10.3892/mmr.2020.11722

Scutellarin regulates osteoarthritis in vitro by inhibiting the PI3K/AKT/mTOR signaling pathway

Authors:
- Shao-Hua Ju
- Li-Rong Tan
- Pan-Wang Liu
- You-Li Tan
- Yuan-Ting Zhang
- Xiao-Hong Li
- Ming-Jian Wang
- Ben-Xiang He
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Affiliations: Department of Pharmacy, Sport Hospital Attached of CDSU, Chengdu Sport University, Chengdu, Sichuan 610041, P.R. China, Department of Internal Medicine, An Jing Countryside Hospital, Chengdu, Sichuan 611730, P.R. China, Department of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China
Published online on: November 23, 2020 https://doi.org/10.3892/mmr.2020.11722
Article Number: 83

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Abstract

Osteoarthritis (OA) is a highly prevalent disease worldwide that causes disability and diminishes the quality of life of affected individuals. The disease is characterized by cartilage destruction, increased inflammatory responses and cholesterol metabolic disorder. Scutellarin is the major active ingredient extracted from Erigeron breviscapus, and it has been demonstrated to possess various pharmacological functions in the treatment of the disease. However, its effects on OA are complex. The present study investigated whether scutellarin can mediate the release of inflammatory cytokines, the expression of collagen- and cholesterol-related proteins, and regulate the phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) signaling pathway in a cell model of OA. Interleukin (IL)-1β was used to stimulate OA in SW1353 cells in vitro. The primary methods used were ELISA and western blotting, which were carried out to examine the effects of scutellarin on the cell model of OA. It was found that scutellarin increased the expression of collagen II and SRY-box 9, whereas it suppressed the expression of matrix metalloproteinase 13. In addition, scutellarin downregulated the expression levels of cholesterol 25-hydroxylase and cytochrome P450 family 7 subfamily B polypeptide 1, but upregulated the expression of apolipoprotein A-1 and adenosine triphosphate-binding cassette transporter A1. The IL-1β-induced increase in the expression of IL-6 was decreased by treatment with scutellarin; however, scutellarin did not alter the expression of C-reactive protein and tumor necrosis factor-α. The protein expression levels of AKT, phosphorylated (p)-AKT, mTOR and p-mTOR in the PI3K/AKT/mTOR signaling pathway were decreased in the IL-1β-induced SW1353 cells following scutellarin treatment. Overall, the findings of the present study demonstrated that scutellarin regulated OA in vitro by inhibiting the PI3K/AKT/mTOR signaling pathway.

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