Open Access

Phgdh serves a protective role in Il‑1β induced chondrocyte inflammation and oxidative‑stress damage

  • Authors:
    • Hefei Huang
    • Keting Liu
    • Hua Ou
    • Xuankun Qian
    • Jianshan Wan
  • View Affiliations

  • Published online on: April 1, 2021     https://doi.org/10.3892/mmr.2021.12058
  • Article Number: 419
  • Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The primary pathological changes observed in osteoarthritis (OA) involve inflammation and degeneration of chondrocytes. 3‑phosphoglycerate dehydrogenase (Phgdh), a rate‑limiting enzyme involved in the conversion of 3‑phosphoglycerate to serine, serves as a crucial molecular component of cell growth and metabolism. However, its effects on chondrocytes in OA have not been determined. In the present study, a rat model of OA was used to investigate the expression levels of Phgdh in vivo and in vitro. Additionally, the role of Phgdh in extracellular matrix (ECM) synthesis, inflammation, apoptosis and oxidative stress levels of chondrocytes was detected in vitro. Phgdh expression was decreased in OA, and Phgdh overexpression promoted ECM synthesis, decreased levels inflammatory cytokines, such as Il‑6, TNF‑α, a disintegrin and metalloproteinase with thrombospondin motifs 5 and MMP13, and decreased apoptosis. Furthermore, expression of Phgdh effectively increased expression levels of the cellular antioxidant enzymes catalase and superoxide dismutase 1, and decreased the levels of reactive oxygen species in chondrocytes; and this may have been regulated by a Kelch like ECH associated protein 1/nuclear factor erythroid 2‑related factor 2 axis. Taken together, these results suggest that Phgdh may be used to manage the progression of OA.
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June-2021
Volume 23 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Huang H, Liu K, Ou H, Qian X and Wan J: Phgdh serves a protective role in Il‑1β induced chondrocyte inflammation and oxidative‑stress damage. Mol Med Rep 23: 419, 2021.
APA
Huang, H., Liu, K., Ou, H., Qian, X., & Wan, J. (2021). Phgdh serves a protective role in Il‑1β induced chondrocyte inflammation and oxidative‑stress damage. Molecular Medicine Reports, 23, 419. https://doi.org/10.3892/mmr.2021.12058
MLA
Huang, H., Liu, K., Ou, H., Qian, X., Wan, J."Phgdh serves a protective role in Il‑1β induced chondrocyte inflammation and oxidative‑stress damage". Molecular Medicine Reports 23.6 (2021): 419.
Chicago
Huang, H., Liu, K., Ou, H., Qian, X., Wan, J."Phgdh serves a protective role in Il‑1β induced chondrocyte inflammation and oxidative‑stress damage". Molecular Medicine Reports 23, no. 6 (2021): 419. https://doi.org/10.3892/mmr.2021.12058