Open Access

An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level

  • Authors:
    • Miguel A. Ortega
    • Ángel Asúnsolo
    • Oscar Fraile‑Martínez
    • Felipe Sainz
    • Miguel A. Saez
    • Coral Bravo
    • Juan A. De León‑Luis
    • Miguel A. Alvarez‑Mon
    • Santiago Coca
    • Melchor Álvarez‑Mon
    • Julia Buján
    • Natalio García‑Honduvilla
  • View Affiliations

  • Published online on: June 2, 2021     https://doi.org/10.3892/mmr.2021.12195
  • Article Number: 556
  • Copyright: © Ortega et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Chronic venous disease (CVD) is the response to a series of hemodynamic changes in the venous system and the onset of this disease is often triggered by pregnancy. Placental tissue is particularly sensitive to the characteristic changes which occurs in venous hypertension. In this regard, changes in the extracellular matrix (ECM), that occur to adapt to this situation, are fundamental to controlling elastogenesis. Therefore, the aim of the present study was to analyze the changes that occur in the mRNA and protein expression level of proteins related to elastogenesis in the placental villi of women diagnosed with CVD, in the third trimester of pregnancy. An observational, analytical and prospective cohort study was conducted, in which the placenta from 62 women with CVD were compared with that in placenta from 52 women without a diagnosis of CVD. Gene and protein expression levels were analyzed using reverse transcription‑quantitative PCR and immunohistochemistry, respectively. The results showed a significant decrease in the gene and protein expression level of EGFL7 in the placental villi of women with CVD. By contrast, significant increases in the gene and protein expression level of ECM‑related proteins, such as tropoelastin, fibulin 4, fibrillin 1 and members of the lysyl oxidase family (LOX and LOXL‑1) were also found in the placental villi of women with CVD. To the best of our knowledge, the results from the present study showed for the first time that CVD during pregnancy was associated with changes in the mRNA and protein expression level in essential components of the EGFL7‑modulated elastogenesis process in placental villi.
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August-2021
Volume 24 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Ortega MA, Asúnsolo Á, Fraile‑Martínez O, Sainz F, Saez MA, Bravo C, De León‑Luis JA, Alvarez‑Mon MA, Coca S, Álvarez‑Mon M, Álvarez‑Mon M, et al: An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level. Mol Med Rep 24: 556, 2021.
APA
Ortega, M.A., Asúnsolo, Á., Fraile‑Martínez, O., Sainz, F., Saez, M.A., Bravo, C. ... García‑Honduvilla, N. (2021). An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level. Molecular Medicine Reports, 24, 556. https://doi.org/10.3892/mmr.2021.12195
MLA
Ortega, M. A., Asúnsolo, Á., Fraile‑Martínez, O., Sainz, F., Saez, M. A., Bravo, C., De León‑Luis, J. A., Alvarez‑Mon, M. A., Coca, S., Álvarez‑Mon, M., Buján, J., García‑Honduvilla, N."An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level". Molecular Medicine Reports 24.2 (2021): 556.
Chicago
Ortega, M. A., Asúnsolo, Á., Fraile‑Martínez, O., Sainz, F., Saez, M. A., Bravo, C., De León‑Luis, J. A., Alvarez‑Mon, M. A., Coca, S., Álvarez‑Mon, M., Buján, J., García‑Honduvilla, N."An increase in elastogenic components in the placental villi of women with chronic venous disease during pregnancy is associated with decreased EGFL7 expression level". Molecular Medicine Reports 24, no. 2 (2021): 556. https://doi.org/10.3892/mmr.2021.12195