Open Access

Polyherbal formulation Anoac‑H suppresses the expression of RANTES and VEGF for the management of bleeding hemorrhoids and fistula

  • Authors:
    • Ashwin Porwal
    • Gopal C. Kundu
    • Gajanan Bhagwat
    • Ramesh Butti
  • View Affiliations

  • Published online on: August 16, 2021     https://doi.org/10.3892/mmr.2021.12376
  • Article Number: 736
  • Copyright: © Porwal et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hemorrhoids and fistula are considered the most common anorectal conditions in the general population. These conditions affect the quality of a patient's life by causing pain and bleeding during defecation or even in the resting state. Lower grades of hemorrhoids may be controlled by traditional measures. However, surgery is an effective treatment option in recurrent‑lower and higher‑grade hemorrhoids. Surgical procedures are associated with various complications, including pain and delayed wound healing. Recurrence of hemorrhoids is also a major concern in the post‑operative period. An anal fistula is the connection between the anus and the skin and causes severe pain, swelling, as well as blood and pus discharge. Fistula has serious social and economic consequences. Hence, it is important to understand the pathophysiology and molecular pathology of hemorrhoids and fistula, to identify the molecular targets and to develop pharmacological‑interventions. In a previous study by our group, the polyherbal formulation Anoac‑H was developed for the treatment of different stages of hemorrhoids and fistula, and it was demonstrated that Anoac‑H is an effective formulation for treating hemorrhoids. However, the molecular mode of action of Anoac‑H on hemorrhoids and fistula had remained elusive. In the present study, it was determined that this formulation reduces the migration of mesenchymal (fibroblasts) and immune (RAW 264.7) cells without affecting their viability. It was also observed that Anoac‑H suppresses the expression of regulated upon activation, normal T cell expressed and presumably secreted (RANTES) and VEGF in fibroblasts and macrophages. Inflammation and elevated expression of RANTES and VEGF were observed in hemorrhoids and fistula. However, inflammation, as well as the expression of RANTES and VEGF, were significantly reduced in treated human hemorrhoid and fistula tissues as compared to untreated ones, confirming the in vitro results.
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October-2021
Volume 24 Issue 4

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Online ISSN:1791-3004

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Spandidos Publications style
Porwal A, Kundu GC, Bhagwat G and Butti R: Polyherbal formulation Anoac‑H suppresses the expression of RANTES and VEGF for the management of bleeding hemorrhoids and fistula. Mol Med Rep 24: 736, 2021.
APA
Porwal, A., Kundu, G.C., Bhagwat, G., & Butti, R. (2021). Polyherbal formulation Anoac‑H suppresses the expression of RANTES and VEGF for the management of bleeding hemorrhoids and fistula. Molecular Medicine Reports, 24, 736. https://doi.org/10.3892/mmr.2021.12376
MLA
Porwal, A., Kundu, G. C., Bhagwat, G., Butti, R."Polyherbal formulation Anoac‑H suppresses the expression of RANTES and VEGF for the management of bleeding hemorrhoids and fistula". Molecular Medicine Reports 24.4 (2021): 736.
Chicago
Porwal, A., Kundu, G. C., Bhagwat, G., Butti, R."Polyherbal formulation Anoac‑H suppresses the expression of RANTES and VEGF for the management of bleeding hemorrhoids and fistula". Molecular Medicine Reports 24, no. 4 (2021): 736. https://doi.org/10.3892/mmr.2021.12376