Open Access

miR‑486‑5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9

  • Authors:
    • Weiwei Wei
    • Chunyu Liu
    • Rongrong Yao
    • Qingyun Tan
    • Qingdong Wang
    • Hao Tian
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  • Published online on: September 3, 2021     https://doi.org/10.3892/mmr.2021.12411
  • Article Number: 771
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Non‑coding RNAs serve essential roles in regulating mRNA and protein expression and dysregulation of non‑coding RNAs participates in a variety of types of cancer. microRNAs (miRNAs/miRs), which are 21‑24 nucleotides non‑coding RNAs, have been shown to be important for the development of gastric cancer (GC). However, the role of miR‑486‑5p in GC remains to be elucidated. The present study found that miR‑486‑5p was downregulated in GC tissues. Comparing with gastric normal cells GES‑1, GC cells, including MKN‑45, AGS, HGC27 and MKN74, had reduced abundance of miR‑486‑5p transcript. CCK8 and colony formation assays demonstrated that GC cell growth and proliferation were enhanced by miR‑486‑5p inhibitors and were suppressed by miR‑486‑5p mimics. miR‑486‑5p also suppressed cell cycle process and migration and promoted apoptosis in GC cells, as verified by propidium iodide (PI) staining, Transwell assay and PI/Annexin V staining. miR‑486‑5p downregulated fibroblast growth factor 9 (FGF9) through combining to its 3'untranslated region. Overexpression of FGF9 accelerated the growth and proliferation of GC cells. The expression of miR‑486‑5p was negatively associated with FGF9 mRNA expression in GC samples. These results revealed that miR‑486‑5p was a tumor suppressor in GC. Downregulation of FGF9 contributed to the role of miR‑486‑5p in GC.
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November-2021
Volume 24 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wei W, Liu C, Yao R, Tan Q, Wang Q and Tian H: miR‑486‑5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9. Mol Med Rep 24: 771, 2021
APA
Wei, W., Liu, C., Yao, R., Tan, Q., Wang, Q., & Tian, H. (2021). miR‑486‑5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9. Molecular Medicine Reports, 24, 771. https://doi.org/10.3892/mmr.2021.12411
MLA
Wei, W., Liu, C., Yao, R., Tan, Q., Wang, Q., Tian, H."miR‑486‑5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9". Molecular Medicine Reports 24.5 (2021): 771.
Chicago
Wei, W., Liu, C., Yao, R., Tan, Q., Wang, Q., Tian, H."miR‑486‑5p suppresses gastric cancer cell growth and migration through downregulation of fibroblast growth factor 9". Molecular Medicine Reports 24, no. 5 (2021): 771. https://doi.org/10.3892/mmr.2021.12411