Role of Thrombospondin‑1 in sepsis‑induced myocardial injury Corrigendum in /10.3892/mmr.2023.13000

Xie,Yun; Zhang,Jiaxiang; Jin,Wei; Tian,Rui; Wang,Ruilan

doi:10.3892/mmr.2021.12509

Role of Thrombospondin‑1 in sepsis‑induced myocardial injury

Corrigendum in: /10.3892/mmr.2023.13000

Authors:
- Yun Xie
- Jiaxiang Zhang
- Wei Jin
- Rui Tian
- Ruilan Wang
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Affiliations: Department of Critical Care Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201600, P.R. China
Published online on: October 25, 2021 https://doi.org/10.3892/mmr.2021.12509
Article Number: 869

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Abstract

Sepsis often causes myocardial injury with a high mortality. The aim of the present study was to investigate the effects of thrombospondin‑1 (THBS1) on myocardial cell injury, oxi‑dative stress and apoptosis in sepsis. The expression of THBS1 mRNA in lipopolysaccharide (LPS)‑induced mouse primary cardiomyocytes was detected by reverse transcription‑quantitative PCR (RT‑qPCR). A eukaryotic small interfering (si)RNA expression vector was constructed and transfected into myocardial cells to knockdown THBS1 mRNA expression, which was confirmed by RT‑qPCR. Four in vitro experimental groups were used: i) Control, ii) LPS, iii) THBS1 siRNA (siTHBS1) and iv) siTHBS + LPS. ELISA was used to detect cardiac troponin I (cTnI), pro‑brain natriuretic peptide (proBNP), reactive oxygen species (ROS), caspase‑3, IL‑6 and TNF‑α. In vivo mouse sepsis models were also established, and H&E, TUNEL and caspase‑3 staining were used to evaluate myocardial cell injury and apoptosis. Clinical samples were collected to analyze the serum THBS1 levels and to associate this with the prognosis of patients with sepsis‑induced myocardial injury. The expression level of THBS1 mRNA in myocardial cells induced by LPS was increased, and the serum THBS1 level in patients with myocardial injury in sepsis was also significantly increased compared with patients without sepsis‑induced myocardial injury. In the siTHBS1‑treated mice with myocardial injury, the levels of cTnI and proBNP were significantly decreased, the levels of the inflammatory cytokines IL‑6 and TNF‑α were significantly decreased, ROS were significantly decreased, caspase‑3 was significantly decreased, and myocardial cell apoptosis was also reduced, compared with the LPS group. Data from the present study suggested that THBS1 may be closely related to the biological behavior of myocardial cells and may be a therapeutic target for myocardial injury in sepsis.

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