Protective effect of H<sub>2</sub>S on LPS‑induced AKI by promoting autophagy

Li,Ting; Zhao,Jie; Miao,Shuying; Chen,Yiyang; Xu,Yunfei; Liu,Ying

doi:10.3892/mmr.2022.12612

Protective effect of H₂S on LPS‑induced AKI by promoting autophagy

Authors:
- Ting Li
- Jie Zhao
- Shuying Miao
- Yiyang Chen
- Yunfei Xu
- Ying Liu
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Affiliations: Department of Physiology, Changzhi Medical College, Changzhi, Shanxi 046000, P.R. China, Department of Neurosurgery, Xiangya Hospital, Central South University, Changsha, Hunan 410078, P.R. China, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410078, P.R. China, Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, Hunan 410078, P.R. China
Published online on: January 20, 2022 https://doi.org/10.3892/mmr.2022.12612
Article Number: 96
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study explored the protective effect of exogenous hydrogen sulfide (H₂S) on lipopolysaccharide (LPS)‑induced acute kidney injury (AKI) and the underlying mechanisms. To establish an AKI injury mouse model, LPS (10 mg/kg) was intraperitoneally injected into mice pretreated with 0.8 mg/kg sodium hydrosulfide hydrate (NaHS), an H₂S donor. The mouse survival rate and the degree of kidney injury were examined. To construct a cell damage model, HK‑2 cells were pretreated with different concentrations (0.1, 0.3 and 0.5 mM) of NaHS, and then the cells were stimulated with LPS (1 µg/ml). The cell viability, autophagy, apoptosis levels and the release of inflammatory factors were examined in mouse kidney tissue and HK‑2 renal tubular epithelial cells. It was found that pretreatment with NaHS significantly improved the survival rate of septic AKI mice, and reduced the renal damage, release of inflammatory factors and apoptosis. In HK‑2 cells, NaHS protected cells from LPS caused damage via promoting autophagy and inhibiting apoptosis and the release of inflammatory factors. In order to clarify the relationship between autophagy and apoptosis and inflammatory factors, this study used 3‑methyladenine (3‑MA) to inhibit autophagy. The results revealed that 3‑MA eliminated the protective effect of NaHS in HK‑2 cells and AKI mice. Overall, NaHS can protect from LPS‑induced AKI by promoting autophagy and inhibiting apoptosis and the release of inflammatory factors.

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