Brucella melitensis outer membrane protein 25 interacts with ferritin heavy polypeptide 1 in human trophoblast cells

Zhang,Yu; Wang,Xiaofeng; Li,Zhiqiang; Zhang,Jing; Wang,Yong; Wu,Changxin; Chen,Chuangfu; Li,Jie; Zhang,Hui

doi:10.3892/mmr.2022.12740

Brucella melitensis outer membrane protein 25 interacts with ferritin heavy polypeptide 1 in human trophoblast cells

Authors:
- Yu Zhang
- Xiaofeng Wang
- Zhiqiang Li
- Jing Zhang
- Yong Wang
- Changxin Wu
- Chuangfu Chen
- Jie Li
- Hui Zhang
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Affiliations: College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, P.R. China, College of Biology and Food, Shangqiu Normal University, Shangqiu, Henan 476000, P.R. China, Institute of Biomedical Sciences, Shanxi University, Taiyuan, Shanxi 030006, P.R. China
Published online on: May 20, 2022 https://doi.org/10.3892/mmr.2022.12740
Article Number: 224

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Abstract

Outer membrane protein 25 (OMP25) is involved in Brucella virulence and serves an important role in Brucella pathogenesis during infection. However, the protein interacting with OMP25 in host cells remains to be elucidated. The present study constructed a cDNA library from Brucella melitensis 16M‑infected human placenta trophoblastic cells (HPT‑8) and identified and confirmed the interaction between Brucella OMP25 and ferritin heavy polypeptide 1 (FTH1) of HPT‑8 using yeast two‑hybrid and co‑immunoprecipitation technologies. The expression of Toll‑like receptor (TLR)4, myeloid differentiation primary response protein MyD88 (MyD88) mRNA and inflammatory factors was detected by RNA interference. The results showed that OMP25 interacted with FTH1. Subsequently, TLR4 and MyD88 mRNA expression levels and inflammatory factors, such as nitric oxide, lactate dehydrogenase and TNF‑α, significantly increased after inserting OMP25 into the HPT‑8 cells. Notably, a low dose of OMP25 resulted in immunological protection, whereas high dose of OMP25 resulted in a cytotoxic effect on the HPT‑8 cells. It is suggested that OMP25 and FTH1 serve important roles in intracellular parasitism of Brucella and inhibition of expression.

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