Open Access

The epigenetic impact of suberohydroxamic acid and 5‑Aza‑2'‑deoxycytidine on DNMT3B expression in myeloma cell lines differing in IL‑6 expression

  • Authors:
    • Katerina Smesny Trtkova
    • Petra Luzna
    • Denisa Weiser Drozdkova
    • Katerina Cizkova
    • Lucie Janovska
    • Jan Gursky
    • Dana Prukova
    • Ivo Frydrych
    • Marian Hajduch
    • Jiri Minarik
  • View Affiliations

  • Published online on: August 29, 2022     https://doi.org/10.3892/mmr.2022.12837
  • Article Number: 321
  • Copyright: © Smesny Trtkova et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gene inactivation of the cyclin‑dependent kinase inhibitors p16INK4a, p15INK4b and p21WAF is frequently mediated by promoter gene methylation, whereas histone deacetylases (HDACs) control gene expression through their ability to deacetylate proteins. The effect of suberohydroxamic acid (SBHA) and 5‑Aza‑2'‑deoxycytidine (Decitabine) (DAC) treatments on the transcription of CDKN2A, CDKN2B and CDKN1A genes, and their effects on molecular biological behavior were examined in two myeloma cell lines, RPMI8226 and U266, which differ in p53‑functionality and IL‑6 expression. In both tested myeloma cell lines, a non‑methylated state of the CDKN2B gene promoter region was detected with normal gene expression, and the same level of p15INK4b protein was detected by immunocytochemical staining. Furthermore, in myeloma cells treated with SBHA and DAC alone, the expression of both p15INK4b and p21WAF was significantly upregulated in RPMI8226 cells (p53‑functional, without IL‑6 expression), whereas in the U266 cell line (p53 deleted, expressing IL‑6) only p21WAF expression was significantly increased. Moreover, the analysis revealed that treatment with DAC induced DNMT3B enhancement in U266 cells. In conclusion, in myeloma cells with IL‑6 expression, significantly increased DNMT3B expression indicated the tumorigenic consequences of 5‑Aza‑2'deoxycytidine treatment, which requires careful use in diseases involving epigenetic dysregulation, such as multiple myeloma (MM).
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October-2022
Volume 26 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Smesny Trtkova K, Luzna P, Drozdkova DW, Cizkova K, Janovska L, Gursky J, Prukova D, Frydrych I, Hajduch M, Minarik J, Minarik J, et al: The epigenetic impact of suberohydroxamic acid and 5‑Aza‑2'‑deoxycytidine on DNMT3B expression in myeloma cell lines differing in IL‑6 expression. Mol Med Rep 26: 321, 2022.
APA
Smesny Trtkova, K., Luzna, P., Drozdkova, D.W., Cizkova, K., Janovska, L., Gursky, J. ... Minarik, J. (2022). The epigenetic impact of suberohydroxamic acid and 5‑Aza‑2'‑deoxycytidine on DNMT3B expression in myeloma cell lines differing in IL‑6 expression. Molecular Medicine Reports, 26, 321. https://doi.org/10.3892/mmr.2022.12837
MLA
Smesny Trtkova, K., Luzna, P., Drozdkova, D. W., Cizkova, K., Janovska, L., Gursky, J., Prukova, D., Frydrych, I., Hajduch, M., Minarik, J."The epigenetic impact of suberohydroxamic acid and 5‑Aza‑2'‑deoxycytidine on DNMT3B expression in myeloma cell lines differing in IL‑6 expression". Molecular Medicine Reports 26.4 (2022): 321.
Chicago
Smesny Trtkova, K., Luzna, P., Drozdkova, D. W., Cizkova, K., Janovska, L., Gursky, J., Prukova, D., Frydrych, I., Hajduch, M., Minarik, J."The epigenetic impact of suberohydroxamic acid and 5‑Aza‑2'‑deoxycytidine on DNMT3B expression in myeloma cell lines differing in IL‑6 expression". Molecular Medicine Reports 26, no. 4 (2022): 321. https://doi.org/10.3892/mmr.2022.12837