Open Access

IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages

  • Authors:
    • Wanying Tang
    • Ming Zhang
    • Yu Wang
    • Dan Ma
    • Mi Hu
    • Yangkai Zhang
    • Huiling Lin
    • Weiwei Jiang
    • Yuxin Ouyang
    • Liping Jiang
    • Pingping He
    • Guojun Zhao
    • Xinping Ouyang
  • View Affiliations

  • Published online on: November 1, 2023     https://doi.org/10.3892/mmr.2023.13121
  • Article Number: 234
  • Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Insulin growth factor‑1 (IGF‑1) is an endocrine regulator that plays an important role in normal growth and development. IGF‑1 mediated effects may result in protecting macrophages from immunometabolic response. However, it is unclear whether IGF‑1 has a protective effect on fatty acid‑induced macrophages damage. In the present study, THP‑1 cells were differentiated into macrophages and stimulated with palmitic acid (PA) in the absence or presence of IGF‑1. Macrophages apoptosis was measured by Cell Counting Kit‑8 assay, flow cytometry, Hoechst 33342 staining and western blotting. The mitochondrial damage was evaluated using JC‑1 staining and mitochondrial reactive oxygen species detection. The activation of mitophagy was assessed using immunofluorescence and western blotting. As a result, IGF‑1 significantly restored the survival rate in macrophages, while the apoptosis was inhibited through mitochondrial pathway. In addition, IGF‑1 protected the mitochondrial damage induced by PA. Furthermore, PA induced mitophagy via phosphatase and tensin homolog‑induced putative kinase protein 1/Parkin, which was reversed by IGF‑1. Taken together, the present study demonstrated the protective effect of IGF‑1 on PA‑induced mitochondrial apoptosis in macrophages, which might provide a potential therapeutic strategy for treatment of lipotoxicity.
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December-2023
Volume 28 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Tang W, Zhang M, Wang Y, Ma D, Hu M, Zhang Y, Lin H, Jiang W, Ouyang Y, Jiang L, Jiang L, et al: IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages. Mol Med Rep 28: 234, 2023
APA
Tang, W., Zhang, M., Wang, Y., Ma, D., Hu, M., Zhang, Y. ... Ouyang, X. (2023). IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages. Molecular Medicine Reports, 28, 234. https://doi.org/10.3892/mmr.2023.13121
MLA
Tang, W., Zhang, M., Wang, Y., Ma, D., Hu, M., Zhang, Y., Lin, H., Jiang, W., Ouyang, Y., Jiang, L., He, P., Zhao, G., Ouyang, X."IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages". Molecular Medicine Reports 28.6 (2023): 234.
Chicago
Tang, W., Zhang, M., Wang, Y., Ma, D., Hu, M., Zhang, Y., Lin, H., Jiang, W., Ouyang, Y., Jiang, L., He, P., Zhao, G., Ouyang, X."IGF‑1 inhibits palmitic acid‑induced mitochondrial apoptosis in macrophages". Molecular Medicine Reports 28, no. 6 (2023): 234. https://doi.org/10.3892/mmr.2023.13121