Effect of fullerenol C60 on lung and renal tissue in lower extremity ischemia‑reperfusion injury in sevoflurane‑treated rats

Arpaci,Ayşe Hande; Köksal,Zeynep; Yiğman,Zeynep; Küçük,Ayşegül; Şivgin,Volkan; Arslan,Mustafa; Kavutçu,Mustafa; Akarca Di̇zakar,Saadet Özen

doi:10.3892/mmr.2024.13178

Effect of fullerenol C60 on lung and renal tissue in lower extremity ischemia‑reperfusion injury in sevoflurane‑treated rats

Authors:
- Ayşe Hande Arpaci
- Zeynep Köksal
- Zeynep Yiğman
- Ayşegül Küçük
- Volkan Şivgin
- Mustafa Arslan
- Mustafa Kavutçu
- Saadet Özen Akarca Di̇zakar
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Affiliations: Department of Anesthesiology and Reanimation, Ankara Training and Research Hospital, Ankara 06230, Turkey, Department of Anesthesiology and Reanimation, Haymana State Hospital, Ankara 06860, Turkey, Department of Histology and Embryology, Faculty of Medicine, Gazi University, Ankara 06510, Turkey, Department of Physiology, Faculty of Medicine, Kutahya Health Sciences University, Kutahya 43020, Turkey, Department of Anesthesiology and Reanimation, Faculty of Medicine, Gazi University, Ankara 06510, Turkey, Department of Medical Biochemistry, Faculty of Medicine, Gazi University, Ankara 06510, Turkey, Department of Histology and Embryology, Faculty of Medicine, İzmir Bakırçay University, İzmir 35665, Turkey
Published online on: February 7, 2024 https://doi.org/10.3892/mmr.2024.13178
Article Number: 54
Copyright: © Arpaci et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to examine the effect of fullerenol C60 on lung and kidney tissue in sevoflurane‑treated rats with lower extremity ischemia‑reperfusion (IR) injury. A total of 30 Wistar albino rats weighing 225‑275 g were used and were equally divided into five groups (n=6/group): i) Sham; ii) IR; iii) IR‑fullerenol C60 (IR‑FUL); iv) IR‑sevoflurane; and v) IR‑fullerenol C60‑sevoflurane (IR‑FUL‑SEVO). Fullerenol C60 was administered intraperitoneally prior to lower extremity IR induction and sevoflurane was administered during the IR injury. Subsequently, lung and kidney histopathological examinations, and serum biochemical analyses were performed. Lung tissue showed markedly increased congestion and neutrophil infiltration in the IR group compared with in the sham group, and notable decreases in congestion and neutrophil infiltration were observed in the treatment groups compared with in the IR group. In the histopathological evaluation of the kidney samples, vacuolization, loss of brush border in tubular epithelial cells, tubular epithelial loss and varying degrees of tubular damage were observed in all groups that underwent IR. There was a significant increase in the mean renal tubule injury score in all IR groups compared with that in the sham group. In addition, the mean kidney injury score was significantly lower in the IR‑FUL and IR‑FUL‑SEVO groups than that in the IR group. It was observed that the expression levels of tumor necrosis factor‑α, interleukin 1β and intercellular adhesion molecule 1 in the lung and kidney tissues were increased following IR, and were decreased in the groups treated with fullerenol C60 and sevoflurane. Notably, it was determined that the reduction in cytokine expression was greatest in the IR‑FUL group. When the oxidant status parameters in the lungs and kidneys were examined, thiobarbituric acid reactive substances levels, and catalase and glutathione S‑transferase enzyme activities were significantly different in the groups receiving sevoflurane or fullerenol C60 treatment compared with those in the IR group. The present study demonstrated the protective effects of fullerenol C60 on the lung and kidney tissues of rats under sevoflurane anesthesia after establishment of lower extremity IR. The results of the present study showed that fullerenol C60 can reduce oxidative and histopathological damage in the lungs and kidneys following IR of the lower extremities.

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