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Dual‑regulated oncolytic adenovirus carrying ERCC1‑siRNA gene possesses potent antitumor effect on ovarian cancer cells

  • Authors:
    • Ting Zhao
    • Wei Ye
    • Rui Zhang
    • Xiaoyan Zhu
    • Qin Shi
    • Xiaofeng Xu
    • Weifeng Chen
    • Ling Xu
    • Yaping Meng
  • View Affiliations

  • Published online on: May 15, 2024     https://doi.org/10.3892/mmr.2024.13245
  • Article Number: 120
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ovarian cancer is a multifactorial and deadly disease. Despite significant advancements in ovarian cancer therapy, its incidence is on the rise and the molecular mechanisms underlying ovarian cancer invasiveness, metastasis and drug resistance remain largely elusive, resulting in poor prognosis. Oncolytic viruses armed with therapeutic transgenes of interest offer an attractive alternative to chemical drugs, which often face innate and acquired drug resistance. The present study constructed a novel oncolytic adenovirus carrying ERCC1 short interfering (si)RNA, regulated by hTERT and HIF promoters, termed Ad‑siERCC1. The findings demonstrated that this oncolytic adenovirus effectively inhibits the proliferation, migration and invasion of ovarian cancer cells. Furthermore, the downregulation of ERCC1 expression by siRNA ameliorates drug resistance to cisplatin (DDP) chemotherapy. It was found that Ad‑siERCC1 blocks the cell cycle in the G1 phase and enhances apoptosis through the PI3K/AKT‑caspase‑3 signaling pathways in SKOV3 cells. The results of the present study highlighted the critical effect of oncolytic virus Ad‑siERCC1 in inhibiting the survival of ovarian cancer cells and increasing chemotherapy sensitivity to DDP. These findings underscore the potent antitumor effect of Ad‑siERCC1 on ovarian cancers in vivo.
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July-2024
Volume 30 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhao T, Ye W, Zhang R, Zhu X, Shi Q, Xu X, Chen W, Xu L and Meng Y: Dual‑regulated oncolytic adenovirus carrying <em>ERCC1</em>‑siRNA gene possesses potent antitumor effect on ovarian cancer cells. Mol Med Rep 30: 120, 2024
APA
Zhao, T., Ye, W., Zhang, R., Zhu, X., Shi, Q., Xu, X. ... Meng, Y. (2024). Dual‑regulated oncolytic adenovirus carrying <em>ERCC1</em>‑siRNA gene possesses potent antitumor effect on ovarian cancer cells. Molecular Medicine Reports, 30, 120. https://doi.org/10.3892/mmr.2024.13245
MLA
Zhao, T., Ye, W., Zhang, R., Zhu, X., Shi, Q., Xu, X., Chen, W., Xu, L., Meng, Y."Dual‑regulated oncolytic adenovirus carrying <em>ERCC1</em>‑siRNA gene possesses potent antitumor effect on ovarian cancer cells". Molecular Medicine Reports 30.1 (2024): 120.
Chicago
Zhao, T., Ye, W., Zhang, R., Zhu, X., Shi, Q., Xu, X., Chen, W., Xu, L., Meng, Y."Dual‑regulated oncolytic adenovirus carrying <em>ERCC1</em>‑siRNA gene possesses potent antitumor effect on ovarian cancer cells". Molecular Medicine Reports 30, no. 1 (2024): 120. https://doi.org/10.3892/mmr.2024.13245