Deciphering the spatiotemporal transcriptional landscape of intestinal diseases (Review)

Guo,Yajing; Ren,Chao; He,Yuxi; Wu,Yue; Yang,Xiaojun

doi:10.3892/mmr.2024.13281

Deciphering the spatiotemporal transcriptional landscape of intestinal diseases (Review)

Authors:
- Yajing Guo
- Chao Ren
- Yuxi He
- Yue Wu
- Xiaojun Yang
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Affiliations: School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 610075, P.R. China, Graduate School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan 410208, P.R. China, Department of Digestive Medicine, Chongqing City Hospital of Traditional Chinese Medicine, Chongqing 400021, P.R. China
Published online on: July 4, 2024 https://doi.org/10.3892/mmr.2024.13281
Article Number: 157
Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The intestines are the largest barrier organ in the human body. The intestinal barrier plays a crucial role in maintaining the balance of the intestinal environment and protecting the intestines from harmful bacterial invasion. Single‑cell RNA sequencing technology allows the detection of the different cell types in the intestine in two dimensions and the exploration of cell types that have not been fully characterized. The intestinal mucosa is highly complex in structure, and its proper functioning is linked to multiple structures in the proximal‑distal intestinal and luminal‑mucosal axes. Spatial localization is at the core of the efforts to explore the interactions between the complex structures. Spatial transcriptomics (ST) is a method that allows for comprehensive tissue analysis and the acquisition of spatially separated genetic information from individual cells, while preserving their spatial location and interactions. This approach also prevents the loss of fragile cells during tissue disaggregation. The emergence of ST technology allows us to spatially dissect enzymatic processes and interactions between multiple cells, genes, proteins and signals in the intestine. This includes the exchange of oxygen and nutrients in the intestine, different gradients of microbial populations and the role of extracellular matrix proteins. This regionally precise approach to tissue studies is gaining more acceptance and is increasingly applied in the investigation of disease mechanisms related to the gastrointestinal tract. Therefore, this review summarized the application of ST in gastrointestinal diseases.

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