Effects of STAT4 on myocardial ischemia‑reperfusion injury and the underlying mechanisms

He,Mei; Yu,Yuexin; Ning,Shuwei; Han,Jingxian; Guo,Zhikun

doi:10.3892/mmr.2024.13321

Effects of STAT4 on myocardial ischemia‑reperfusion injury and the underlying mechanisms

Authors:
- Mei He
- Yuexin Yu
- Shuwei Ning
- Jingxian Han
- Zhikun Guo
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Affiliations: Henan Key Laboratory of Cardiac Remodeling and Transplantation, Zhengzhou No. 7 People's Hospital, Zhengzhou, Henan 450016, P.R China
Published online on: September 2, 2024 https://doi.org/10.3892/mmr.2024.13321
Article Number: 197
Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The regulation of cardiac function by the nuclear transcription factor signal transducer and activator of transcription 4 (STAT4) has been recently recognized. Nevertheless, the role and mechanisms of action of STAT4 in myocardial ischemia‑reperfusion (I/R) injury remain unknown. Consequently, the present study constructed a rat model of I/R by ligation of the left anterior descending coronary artery. Following sacrifice, the rat hearts were excised and analyzed to investigated the effects of STAT4 on I/R‑induced myocardial injury. Western blotting demonstrated that expression of STAT4 decreased significantly in the rat model of cardiac I/R and in H9C2 cells that were subjected to hypoxia and reoxygenation (H/R). The overexpression of STAT4 in H9C2 cells reduced cell damage and apoptosis induced by H/R. Furthermore, both in vivo and in vitro, the level of PI3K decreased significantly. Although the AKT protein expression levels were not altered, the AKT phosphorylation levels decreased significantly. STAT4 overexpression enhanced the expression of PI3K and AKT in the H9C2 cells. On the whole, the present study demonstrated that STAT4 alleviated I/R‑induced myocardial injury through the PI3K/AKT signaling pathway.

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