Open Access

Stigmasterol exerts antiglioma effects by regulating lipid metabolism

  • Authors:
    • Ting Wei
    • Ruichun Li
    • Shiwen Guo
    • Chen Liang
  • View Affiliations

  • Published online on: October 4, 2024     https://doi.org/10.3892/mmr.2024.13351
  • Article Number: 227
  • Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Stigmasterol is a sterol compound found in various traditional Chinese medicines; however, its effects on glioma remain unclear. The present study aimed to investigate the effects of stigmasterol on the biological behaviors of glioblastoma (GBM) cells and to explore the underlying mechanisms. In vitro experiments assessed its effects on GBM cell proliferation, apoptosis, cell cycle progression, invasion, migration and vasculogenic mimicry (VM). The potential targets for stigmasterol in treating GBM were identified using databases and Venn diagram analysis, followed by enrichment analysis using R language. A prognostic model related to the target genes of stigmasterol was developed through univariate Cox regression and least absolute shrinkage and selection operator analyses. Stigmasterol was found to suppress the proliferation of GBM cells in a dose‑ and time‑dependent manner, to induce apoptosis, and to inhibit invasion, migration and VM formation. Additionally, 31 potential targets of stigmasterol were identified, linked to lipid metabolism and the G protein‑coupled receptor signaling pathway. Lipid metabolism assays revealed that stigmasterol significantly reduced free fatty acids and total cholesterol levels. Furthermore, two prognosis‑related target genes, fatty acid binding protein 5 and α‑1B adrenergic receptor, were selected, and the prognostic model effectively predicted GBM outcomes. Moreover, molecular docking revealed strong binding affinities between stigmasterol and the target proteins. Overall, these findings suggested that stigmasterol may exert anti‑glioma effects, which could be potentially mediated through the regulation of lipid metabolism.
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December-2024
Volume 30 Issue 6

Print ISSN: 1791-2997
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Spandidos Publications style
Wei T, Li R, Guo S and Liang C: Stigmasterol exerts antiglioma effects by regulating lipid metabolism. Mol Med Rep 30: 227, 2024.
APA
Wei, T., Li, R., Guo, S., & Liang, C. (2024). Stigmasterol exerts antiglioma effects by regulating lipid metabolism. Molecular Medicine Reports, 30, 227. https://doi.org/10.3892/mmr.2024.13351
MLA
Wei, T., Li, R., Guo, S., Liang, C."Stigmasterol exerts antiglioma effects by regulating lipid metabolism". Molecular Medicine Reports 30.6 (2024): 227.
Chicago
Wei, T., Li, R., Guo, S., Liang, C."Stigmasterol exerts antiglioma effects by regulating lipid metabolism". Molecular Medicine Reports 30, no. 6 (2024): 227. https://doi.org/10.3892/mmr.2024.13351