Copy number changes of CRISP3 in oral squamous cell carcinoma

Ko,Wen-Chang; Sugahara,Keisuke; Sakuma,Takumi; Yen,Ching-Yu; Liu,Shyun-Yeu; Liaw,Gwo-An; Shibahara,Takahiko

doi:10.3892/ol.2011.418

Copy number changes of CRISP3 in oral squamous cell carcinoma

Authors:
- Wen-Chang Ko
- Keisuke Sugahara
- Takumi Sakuma
- Ching-Yu Yen
- Shyun-Yeu Liu
- Gwo-An Liaw
- Takahiko Shibahara
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Affiliations: Department of Oral and Maxillofacial Surgery, Tokyo Dental College, Mihama, Chiba 261-8502, Japan, Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Yongkang, Taiwan, R.O.C.
Published online on: September 9, 2011 https://doi.org/10.3892/ol.2011.418
Pages: 75-81

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Abstract

The aim of this study was to identify tumor suppressor genes (TSGs) in oral squamous cell carcinoma (OSCC) using whole-genome analysis of microarray technology and real-time quantitative polymerase chain reaction (QPCR). We applied whole-genome analysis of TSGs in the specimens from 3 patients of OSCC by microarray technology. A total of 11 genes, CRISP3, SCGB3A1, AGR2, PIP, C20orf114, TFF1, STATH, AZGP1, MUC7, DMBT1 and LOC389429, were found to be down-regulated, and 2, matrix metallopeptidase (MMP) 1 and MMP3, were found to be up-regulated in the 3 OSCC patients using microarray technology. In this study, we selected the CRISP3 gene. CRISP3 belongs to the cystein-rich secretary protein gene family in chromosome 6p12.3. CRISP3 has been found in the salivary gland, spleen and prostate gland and is a prominent biomarker in the gene expression of prostate cancer. Down-regulation of this gene was previously observed in OSCC. No studies examining the DNA copy number of CRISP3 in detail exist. We analyzed the DNA copy number of CRISP3 in 5 OSCC-derived cell lines (SAS, Ca9-22, KON, HSC2 and HSC4) and 60 OSCC tissues by real-time QPCR. The DNA copy number loss of CRISP3 was observed in 2 of the 5 OSCC-derived cell lines (SAS, HSC2) and in 24 of 60 patients (40.0%) using real-time QPCR. A significant statistical correlation between the copy number loss and gender and T classification was observed. These results indicate that the inactivation of CRISP3 is an early event in OSCC, since the T1/T2 classification is correlated with DNA copy number loss of CRISP3, whereas T3/T4 classification is not. We conclude that CRISP3 may be involved in the carcinogenesis of OSCC.

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