Protein expression pattern in response to ionizing radiation in MCF-7 human breast cancer cells

Jung,Samil; Lee,Soonduck; Lee,Jayhee; Li,ChenGping; Ohk,Ji-Yeon; Jeong,Hyeon‑Kyung; Lee,Seungkyu; Kim,Sangwoo; Choi,Yunyeong; Kim,Sunghak; Lee,Heungwoo; Lee,Myeong-Sok

doi:10.3892/ol.2011.444

Protein expression pattern in response to ionizing radiation in MCF-7 human breast cancer cells

Authors:
- Samil Jung
- Soonduck Lee
- Jayhee Lee
- ChenGping Li
- Ji-Yeon Ohk
- Hyeon‑Kyung Jeong
- Seungkyu Lee
- Sangwoo Kim
- Yunyeong Choi
- Sunghak Kim
- Heungwoo Lee
- Myeong-Sok Lee
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Affiliations: Division of Biological Science and Research Center for Women's Diseases, Sookmyung Women's University, Seoul 140-742, Republic of Korea, Seoul Science High School, Seoul, Republic of Korea
Published online on: October 18, 2011 https://doi.org/10.3892/ol.2011.444
Pages: 147-154

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Abstract

Breast cancer is one of the most common types of cancer in women and is highly treatable by radiotherapy. However, repeated exposure to radiation results in tumor cell resistance. Understanding the molecular mechanisms involved in the response of tumors to γ-irradiation is important for improving radiotherapy. For this reason, we aimed to identify radiation-responsive genes at the protein level. In the present study, we observed differentially expressed proteins using 2D-PAGE and MALDI-TOF-MS for the global analysis of protein expression patterns in response to ionizing radiation (IR). When the expression patterns of proteins were compared to a control gel, numerous spots were found that differed greatly. Among them, 11 spots were found to be significantly different. One set of proteins (GH2, RGS17, BAK1, CCNH, TSG6, RAD51B, IGFBP1 and CASP14) was upregulated and another set of proteins (C1QRF, PLSCR2 and p34SE1-1) was downregulated after exposure to γ-rays. These proteins are known to be related to cell cycle control, apoptosis, DNA repair, cell proliferation and other signaling pathways.

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1	El-Deiry WS: The role of p53 in chemosensitivity and radio-sensitivity. Oncogene. 22:7486–7495. 2003. View Article : Google Scholar : PubMed/NCBI
2	Iliakis G, Wang Y, Guan J and Wang H: DNA damage checkpoint control in cells exposed to ionizing radiation. Oncogene. 22:5834–5847. 2003. View Article : Google Scholar : PubMed/NCBI
3	Amundson SA, Myers TG and Fornace Jr: Roles for p53 in growth arrest and apoptosis: Putting on the brakes after genotoxic stress. Oncogene. 17:3287–3299. 1998. View Article : Google Scholar : PubMed/NCBI
4	Fornace AJ Jr, Amundson SA, Bittner M, Myers TG, Meltzer P, Weinsten JN and Trent J: The complexity of radiation stress reponse: analysis by informatics and functional genomics approaches. Gene Expr. 7:387–400. 1999.PubMed/NCBI
5	King TC, Estalilla OC and Safran H: Role of p53 and p16 gene alterations in determining response to concurrent paclitaxel and radiation in solid tumor. Semin Radiat Oncol. 9:4–11. 1999.PubMed/NCBI
6	Lu X and Lane DP: Differential induction of transcriptionally active p53 following UV or ionizing irradiation: defects in chromosome instability syndromes? Cell. 75:765–778. 1993. View Article : Google Scholar : PubMed/NCBI
7	Keyse SM: The induction of gene expression in mammalian cells by radiation. Semin Cancer Biol. 4:119–128. 1993.PubMed/NCBI
8	Iliakis G: Cell cycle regulation in irradiated and nonirradiated cells. Semin Oncol. 24:602–615. 1997.PubMed/NCBI
9	Eckardt Schupp F and Klaus C: Radiation inducible DNA repair processes in eukaryotes. Biochimie. 81:161–171. 1999.PubMed/NCBI
10	Maity A, McKenna WG and Muschel RJ: The molecular basis for cell cycle delays following ionizing radiation: a review. Radiother Oncol. 31:1–13. 1994. View Article : Google Scholar : PubMed/NCBI
11	Forrester HB, Vidair CA, Albright N, Ling CC and Dewey WC: Using computerized video time lapse for quantifying cell death of Xirradiated rat embryo cells transfected with cmyc or cHaras. Cancer Res. 59:931–939. 1999.PubMed/NCBI
12	Kasid U, Suy S, Dent P, Ray S, Whiteside TL and Sturgill TW: Activation of Raf by ionizing radiation. Nature. 382:813–816. 1996. View Article : Google Scholar : PubMed/NCBI
13	Waldman T, Lengauer C, Kinzler KW and Vogelstein B: Uncoupling of S phase and mitosis induced by anticancer agents in cells lacking p21. Nature. 381:713–716. 1996. View Article : Google Scholar : PubMed/NCBI
14	El-Deiry WS: Regulation of p53 downstream genes. Semin Cancer Biol. 8:345–357. 1998. View Article : Google Scholar
15	Robson T, Joiner MC, Wilson GD, McCullough W, Price ME, Logan I, Jones H, McKeown SR and Hirst DG: A novel human stress response-related gene with a potential role in induced radio-resistance. Radiat Res. 152:451–461. 1999. View Article : Google Scholar : PubMed/NCBI
16	Lowe SW, Schmitt EM, Smith SW, Osborne BA and Jacks T: p53 is required for radiation-induced apoptosis in mouse thymocytes. Nature. 362:847–849. 1993. View Article : Google Scholar : PubMed/NCBI
17	Honda M, Kaneko S, Kawai H, Shirota Y and Kobayashi K: Differential gene expression between chronic hepatitis B and C hepatic lesion. Gastroenterology. 120:955–966. 2001. View Article : Google Scholar : PubMed/NCBI
18	Okabe H, Satoh S, Kato T, Kitahara O, Yanagawa R, Yamaoka Y, Tsunoda T, Furukawa Y and Nakamura Y: Genome-wide analysis of gene expression in human hepatocellular carcinomas using cDNA microarray: identification of genes involved in viral carcinogenesis and tumor progression. Cancer Res. 61:2129–2137. 2001.
19	Shirota Y, Kaneko S, Honda M, Kawai HF and Kobayshi K: Identification of differently expressed genes in hepatocellular carcinoma with cDNA microarrays. Hepatology. 33:832–840. 2001. View Article : Google Scholar : PubMed/NCBI
20	Takeo S, Arai H, Kusano N, Harada T, et al: Examination of oncogene amplification by genomic DNA microarray in hepatocellular carcinomas comparison with comparative genomic hybridization analysis. Cancer Genet Cytogenet. 130:127–132. 2001. View Article : Google Scholar
21	Xu XR, Huang J, Xu ZG, et al: Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver. Proc Natl Acad Sci USA. 98:15089–15094. 2001. View Article : Google Scholar
22	Kis E, Szatmari T, Keszei M, Farkas R, Esik O, Lumniczky K, Falus A and Safrany G: Microarray analysis of radiation response genes in primary human fibroblasts. Int J Radiat Oncol Biol Phys. 66:1506–1514. 2006. View Article : Google Scholar : PubMed/NCBI
23	Hondermarck H, Vercoutter-Edouart A-S, Revillion F, Lemoine J, El-Yazidi-Belkoura I, Nurcombe V and Peyrat JP: Proteomics of breast cancer for marker discovery and signal pathway profiling. Proteomics. 1:1216–1232. 2001. View Article : Google Scholar : PubMed/NCBI
24	Oh JM, Brichory F, Puravs E, Kuick R, Wood C, Rouillard JM, Tra J, Kardia S, Beer D and Hanash S: A database of protein expression in lung cancer. Proteomics. 1:1303–1319. 2001. View Article : Google Scholar : PubMed/NCBI
25	Stulik J, Hernychova L, Porkertova S, Knižek J, Macela A, Bures J, Jandik P, Langridge JI and Jungblut PR: Proteome study of colorectal carcinogenesis. Electrophoresis. 22:3019–3025. 2001. View Article : Google Scholar : PubMed/NCBI
26	Kim W, Oe LS, Kim JS, Ryu YH, Byeon JY, Kim HJ, Kim YI, Heo JS, Park YM and Jung G: Comparison of proteome between hepatitis B virus- and hepatitis C virus-associated hepatocellular carcinoma. Clin Cancer Res. 9:5493–5500. 2003.PubMed/NCBI
27	Janicke RU, Engels IH, Dunkern T, Kaina B, Schulze-Osthoff K and Porter AG: Ionizing radiation but not anticancer drugs causes cell cycle arrest and failure to activate the mitochondrial death pathway in MCF-7 breast carcinoma cells. Oncogene. 20:5043–5053. 2001. View Article : Google Scholar : PubMed/NCBI
28	Albala JS, Thelen MP, Prange C, Fan W, Christensen M, Thompson LH and Lennon GG: Identification of a novel human RAD51 homolog, RAD51B. Genomics. 46:476–479. 1997. View Article : Google Scholar : PubMed/NCBI
29	Jin K, Nagayama T, Chen J, Stetler AR, Kawaguchi K, Simon RP and Graham SH: Molecular cloning of a cell cycle regulation gene cyclin H from ischemic rat brain: expression in neurons after global cerebral ischemia. J Neurochem. 73:1598–1608. 1999. View Article : Google Scholar : PubMed/NCBI
30	Lee TH, Wisniewski HG and Vilcek J: A novel secretory tumor necrosis factor-inducible protein (TSG-6) is a member of the family of hyaluronate binding proteins, closely related to the adhesion receptor CD44. J Cell Biol. 116:545–557. 1992. View Article : Google Scholar
31	Kaulsay KK, Mertani HC, Tornell J, Morel G, Lee KO and Lobie PE: Autocrine stimulation of human mammary carcinoma cell proliferation by human growth hormone. Exp Cell Res. 250:35–50. 1999. View Article : Google Scholar : PubMed/NCBI
32	Oliveira-dos-Santos AJ, Matsumoto G, Snow BE, et al: Regulation of T cell activation, anxiety, and male aggression by RGS2. Proc Natl Acad Sci USA. 97:12272–12277. 2000. View Article : Google Scholar : PubMed/NCBI
33	Pearson AS, Spitz FR, Swisher SG, Kataoka M, Sarkiss MG, Meyn RE, McDonnell TJ, Cristiano RJ and Roth JA: Up- regulation of the proapoptotic mediators Bax and Bak after adenovirus-mediated p53 gene transfer in lung cancer cells. Clin Cancer Res. 6:887–890. 2000.PubMed/NCBI
34	Chittenden T, Harrington EA, O’Connor R, Flemington C, Lutz RJ, Evan GI and Guild BC: Induction of apoptosis by the Bcl-2 homologue Bak. Nature. 374:733–736. 1995. View Article : Google Scholar : PubMed/NCBI
35	Farrow SN, White JH, Martinou I, Raven T, Pun KT, Grinham CJ, Martinou JC and Brown R: Cloning of a bcl-2 homo-logue by interaction with adenovirus E1B 19K. Nature. 374:731–733. 1995. View Article : Google Scholar : PubMed/NCBI
36	Kiefer MC, Brauer MJ, Powers VC, Wu JJ, Umansky SR, Tomei LD and Barr PJ: Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak. Nature. 374:736–739. 1995. View Article : Google Scholar : PubMed/NCBI
37	Clemmons DR, Camacho-Hubner C, Coronado E and Osborne CK: Insulin-like growth factor binding protein secretion by breast carcinoma cell lines: correlation with estrogen receptor status. Endocrinology. 127:2679–2686. 1990. View Article : Google Scholar : PubMed/NCBI
38	Pekonen F, Nyman T, Ilvesmöki V and Partanen S: Insulin-like growth factor binding proteins in human breast cancer tissue. Cancer Res. 52:5204–5207. 1992.PubMed/NCBI
39	Pistritto G, Jost M, Srinivasula SM, Baffa R, Poyet JL, Kari C, Lazebnik Y, Rodeck U and Alnemri ES: Expression and transcriptional regulation of caspase-14 in simple and complex epithelia. Cell Death Differ. 9:995–1006. 2002. View Article : Google Scholar : PubMed/NCBI
40	Jurianz K, Ziegler S, Garcia-Schüler H, Kraus S, Bohana-Kashtan O, Fishelson Z and Kirschfink M: Complement resistance of tumor cells: basal and induced mechanisms. Mol Immunol. 36:929–939. 1999. View Article : Google Scholar : PubMed/NCBI
41	Sugimoto M, Nakamura T, Ohtani N, Hampson L, Hampson IN, Shimamoto A, Furuichi Y, Okumura K, Niwa S, Taya Y and Hara E: Regulation of CDK4 activity by a novel CDK4-binding protein, p34 (SEI-1). Genes Dev. 13:3027–3033. 1999. View Article : Google Scholar : PubMed/NCBI
42	Herbert BR, Sanchez J-C, Bini L, Wilkins MR, Williams KL, Appel RD and Hochstrasser DF: Proteome Research: New Frontiers in Functional Genomics. Springer; Berlin: pp. 13–33. 1997
43	Sherr CJ and Roberts JM: CDK inhibitors: positive and negative regulators of G1-phase progression. Genes Dev. 13:1501–1512. 1999. View Article : Google Scholar : PubMed/NCBI
44	Bartek J, Bartkova J and Lukas J: The retinoblastoma protein pathway in cell cycle control and cancer. Exp Cell Res. 237:1–6. 1997. View Article : Google Scholar : PubMed/NCBI