Effects of resveratrol on vascular endothelial growth factor expression in osteosarcoma cells and cell proliferation

Liu,Zhonghe; Li,Yanlan; Yang,Rui

doi:10.3892/ol.2012.824

Effects of resveratrol on vascular endothelial growth factor expression in osteosarcoma cells and cell proliferation

Authors:
- Zhonghe Liu
- Yanlan Li
- Rui Yang
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Affiliations: Department of Orthopedics, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China, Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China, Department of Life Science and Technology, Xinxiang Medical University, Xinxiang, Henan 453003, P.R. China
Published online on: July 25, 2012 https://doi.org/10.3892/ol.2012.824
Pages: 837-839

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Abstract

The aim of the current study was to investigate the effects of resveratrol (Res) on vascular endothelial growth factor (VEGF) expression and cell proliferation in the human osteosarcoma cell line U20S. U20S cells were treated with Res at various concentrations (0, 10, 20 and 40 µmol/l) for various times (24, 48 and 72 h). The inhibitory effect of Res on U20S proliferation was observed using methyl thiazolyl tetrazolium (MTT) colorimetry. VEGF expression was determined using real-time polymerase chain reaction (RT-PCR) and western blot analysis. The inhibitory effect of Res on U20S proliferation increased as the concentration of Res increased. The inhibitory effect also increased with time. Res had an inhibitory effect on VEGF expression and significantly inhibited U20S cell proliferation. Res may exert an anti-osteosarcoma effect by inhibiting VEGF expression in tumor cells.

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