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Regulation of neutrophil gelatinase-associated lipocalin expression by C/EBPβ in lung carcinoma cells

  • Authors:
    • Pi-Xian Zhang
    • Jing-Xia Chang
    • Jian-Jun Xie
    • Hua-Min Yuan
    • Ze-Peng Du
    • Fa-Ren Zhang
    • Zhuo Lü
    • Li-Yan Xu
    • En-Min Li

  • View Affiliations

  • Published online on: August 9, 2012     https://doi.org/10.3892/ol.2012.859
  • Pages: 919-924
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Abstract

Neutrophil gelatinase-associated lipocalin (NGAL), a member of the lipocalin family, has been found to be overexpressed in a variety of tumors, including lung adeno­carcinomas. However, the mechanism by which NGAL expression is regulated in lung carcinoma needs further evaluation. In this study, immunohistochemistry was employed to analyze the expression of NGAL in lung carcinoma tissue samples, including lung squamous carcinomas, adenocarcinomas, adenosquamous carcinomas and bronchial alveolar cell carcinomas. The results showed that NGAL was expressed in 82.61% (19/23) of the samples. RT-PCR and immunofluorescent staining showed that NGAL was localized to the cytoplasm in lung carcinoma cell lines. To explore the transcriptional regulation mechanism of NGAL basal expression in lung carcinoma, a 1515-bp fragment (-1431 to +84) of the NGAL promoter region was cloned and a series of deletion and mutation constructs were generated. These constructs were analyzed using the luciferase reporter assay. The results indicated that the cis-acting elements important for the basal activity of NGAL transcription were likely located between -152 and -141. Further analysis using site-directed mutagenesis and the luciferase reporter assay suggested that the C/EBP binding sites were responsible for the activity of the NGAL promoter. Finally, the binding ability and specificity of the transcription factors were determined by electrophoretic mobility-shift assay (EMSA). The results showed that C/EBPβ was able to bind to the -152 and -141 segments. Taken together, these findings suggest that NGAL is expressed in lung carcinomas and that NGAL expression is mediated by the binding of C/EBPβ to the -152 and -141 segment of the NGAL promoter.
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November 2012
Volume 4 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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  • CiteScore: 5.7
  • CiteScore Rank: Q2: #139/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.574
  • SCImago Journal Rank (SJR): 0.644
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Spandidos Publications style
Zhang P, Chang J, Xie J, Yuan H, Du Z, Zhang F, Lü Z, Xu L and Li E: Regulation of neutrophil gelatinase-associated lipocalin expression by C/EBPβ in lung carcinoma cells. Oncol Lett 4: 919-924, 2012.
APA
Zhang, P., Chang, J., Xie, J., Yuan, H., Du, Z., Zhang, F. ... Li, E. (2012). Regulation of neutrophil gelatinase-associated lipocalin expression by C/EBPβ in lung carcinoma cells. Oncology Letters, 4, 919-924. https://doi.org/10.3892/ol.2012.859
MLA
Zhang, P., Chang, J., Xie, J., Yuan, H., Du, Z., Zhang, F., Lü, Z., Xu, L., Li, E."Regulation of neutrophil gelatinase-associated lipocalin expression by C/EBPβ in lung carcinoma cells". Oncology Letters 4.5 (2012): 919-924.
Chicago
Zhang, P., Chang, J., Xie, J., Yuan, H., Du, Z., Zhang, F., Lü, Z., Xu, L., Li, E."Regulation of neutrophil gelatinase-associated lipocalin expression by C/EBPβ in lung carcinoma cells". Oncology Letters 4, no. 5 (2012): 919-924. https://doi.org/10.3892/ol.2012.859