Tagged and untagged TRAIL show different activity against tumor cells
- Authors:
- Kunpeng Zhao
- Yan'ge Wang
- Xueyin Wang
- Yugang Wang
- Yuanfang Ma
View Affiliations
Affiliations: Department of Cell Biology, Institute of Immunology, Medical College of Henan University, Henan 475003, P.R. China, Department of Cell Biology, Institute of Immunology, Medical College of Henan University, Henan 475003, P.R. China, Department of Immunology, Henan Medical College for Staff and Workers, Zhengzhou, Henan 451191, P.R. China, Department of Immunology, Institute of Basic Medical Sciences, Beijing 100850, P.R. China
- Published online on: September 11, 2012 https://doi.org/10.3892/ol.2012.908
-
Pages:
1301-1304
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Abstract
The tumor necrosis factor-related apoptosis‑inducing ligand (TRAIL/Apo2L) is a novel cytotoxic ligand belonging to the TNF superfamily which is currently being developed as a cancer therapeutic drug. Here, we observed the different functions of recombinant TRAIL protein with a foreign protein label and non-labeled TRAIL. We used a prokaryotic expression system to prepare two different versions of the extracellular TRAIL 114-281aa protein: TRAIL-HS, a protein modified with 6xHis-Tag and S-Tag; and TRAIL-FT, which had no foreign protein. The proteins were purified using Ni-NTA chromatography (TRAIL-HS) and cation ion‑exchange column chromatography (TRAIL-FT) and identified by SDS-PAGE and western blot analysis. We compared the abilities of the proteins to bind to death receptor 5 (DR5) by ELISA and to induce apoptosis in a normal liver cell line (Chang liver) and a human T-lymphocyte leukemia cell line (Jurkat) by MTT assay, GR staining and FACS. The results indicate that the biological functions of TRAIL-FT were superior to those of TRAIL-HS in binding and the induction of apoptosis, and may be useful to further the development and applications of TRAIL.
View References
1.
|
SR WileyK SchooleyPJ SmolakIdentification
and characterization of a new member of the TNF family that induces
apoptosisImmunity3673682199510.1016/1074-7613(95)90057-88777713
|
2.
|
H WalczakRE MillerK AriailTumoricidal
activity of tumor necrosis factor-related apoptosis-inducing ligand
in vivoNat Med5157163199910.1038/55179930862
|
3.
|
M LeverkusH WalczakA McLellanMaturation of
dendritic cells leads to up-regulation of cellular FLICE-inhibitory
protein and concomitant down-regulation of death ligand-mediated
apoptosisBlood96262826312000
|
4.
|
H YagitaK TakedaY HayakawaTRAIL and its
receptors as targets for cancer therapyCancer
Sci95777783200410.1111/j.1349-7006.2004.tb02181.x15504243
|
5.
|
M JoTH KimDW SeolJE EsplenApoptosis
induced in normal human hepatocytes by tumor necrosis
factor-related apoptosis-inducing ligandNat
Med6564567200010.1038/7504510802713
|
6.
|
D LawrenceZ ShahrokhS MarstersDifferential
hepatocyte toxicity of recombinant Apo2L/TRAIL versionsNat
Med7383385200110.1038/8639711283636
|
7.
|
A AshkenaziRC PaiS FongSafety and
antitumor activity of recombinant soluble Apo2 ligandJ Clin
Invest104155162199910.1172/JCI692610411544
|
8.
|
E CretneyA ShankerH YagitaTNF-related
apoptosis-inducing ligand as a therapeutic agent in autoimmunity
and cancerImmunol Cell
Biol848798200610.1111/j.1440-1711.2005.01413.x16405656
|
9.
|
JZ QinP BaconV ChaturvediBJ NickoloffRole
of NF-κB activity in apoptotic response of keratinocytes mediated
by interferon-gamma, tumor necrosis factor-α, and tumor necrosis
factor-related apoptosis-inducing ligandJ Invest
Dermatol1178989072001
|
10.
|
GS WuTRAIL as a target in anti-cancer
therapyCancer Lett28515200910.1016/j.canlet.2009.02.02919299078
|
11.
|
M JoTH KimDW SeolApoptosis induced in
normal human hepatocytes by tumor necrosis factor-related
apoptosis-inducing ligandNat
Med6564567200010.1038/7504510802713
|
12.
|
D LawrenceZ ShahrokhS MarstersDifferential
hepatocyte toxicity of recombinant Apo2L/TRAIL versionsNat
Med7383385200110.1038/8639711283636
|
13.
|
AY SunYL ShenJC YinImprovement of
expression level and bioactivity of soluble tumor necrosis
factor-related apoptosis-inducing ligand (Apo2L/TRAIL) by a novel
zinc ion feeding strategyBiotechnol
Lett2812151219200610.1007/s10529-006-9073-z16799759
|