Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines

Ling,Gui-Qin; Chen,Dong-Bo; Wang,Bao-Qing; Zhang,Lan-Sheng

doi:10.3892/ol.2012.916

Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines

Authors:
- Gui-Qin Ling
- Dong-Bo Chen
- Bao-Qing Wang
- Lan-Sheng Zhang
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Affiliations: The Second Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu 221006, P.R.China
Published online on: September 13, 2012 https://doi.org/10.3892/ol.2012.916
Pages: 1264-1268

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Abstract

Previous studies have demonstrated that pluripotency-associated transcription factors, such as Oct3/4, Nanog and Sox2, play a crucial role in the development and malignant progression of various types of tumors. Breast cancer is the most frequent cancer among females, being a heterogeneous disease, with distinct morphologies, metastatic behavior and therapeutic responses. The expression of Oct3/4, Nanog and Sox2 in 3 human breast cancer cell lines, MCF7, T-47D and MDA-MB‑231, was determined. The expression of Oct3/4, Nanog and Sox2 mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR) and protein expression was detected by immunocytohistochemistry. RT-PCR revealed that all three human breast cancer cell lines tested expressed evident Oct3/4, Nanog and Sox-2 mRNA at various levels. Higher levels of Oct3/4 were identified in MCF7 and MDA-MB-231 cells compared with T-47D cells. Higher levels of Nanog were observed in MCF7 and T-47D cells compared with MDA-MB-231 cells and the highest expression of Sox-2 was detected in MCF7 cells. The nuclear localization of Oct3/4, Nanog and Sox-2 was confirmed by immunostaining. Oct3/4, Nanog and Sox2 were expressed in human breast cancer cell lines. Further studies are required to characterize the role of Oct3/4, Nanog and Sox2 in human breast cancer.

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