In vitro and in vivo growth inhibition of drug-resistant ovarian carcinoma cells using a combination of cisplatin and a TRAIL-encoding retrovirus

Li,Fang; Guo,Yi; Han,Lingfei; Duan,Yankang; Fang,Fang; Niu,Sumei; Ba,Qiujie; Zhu,Huaishi; Kong,Fanfei; Lin,Chao; Wen,Xuejun

doi:10.3892/ol.2012.926

In vitro and in vivo growth inhibition of drug-resistant ovarian carcinoma cells using a combination of cisplatin and a TRAIL-encoding retrovirus

Authors:
- Fang Li
- Yi Guo
- Lingfei Han
- Yankang Duan
- Fang Fang
- Sumei Niu
- Qiujie Ba
- Huaishi Zhu
- Fanfei Kong
- Chao Lin
- Xuejun Wen
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Affiliations: Cervical Disease Centre, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 200040, P.R. China, Department of Radiology, Huangpu District Central Hospital, Shanghai 200002, P.R. China, The Institute for Advanced Materials and Nano Biomedicine, Tongji University, Shanghai 200092, P.R. China
Published online on: September 20, 2012 https://doi.org/10.3892/ol.2012.926
Pages: 1254-1258

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Abstract

Retroviruses encoding the TNF-related apoptosis-inducing ligand (TRAIL) gene were generated by transient transfection of the retrovirus packing cell line BOSC 23 using TRAIL-encoding plasmid. The retrovirus was able to transduce drug-resistant A2780/DDP ovarian carcinoma cells in vitro and induce TRAIL expression in the cells, as detected by western blot assay. Furthermore, the TRAIL protein led to the growth inhibition of the cells via a caspase-activated apoptotic mechanism. It was confirmed that exposure of such cells to cisplatin in combination with the TRAIL-encoding retrovirus resulted in higher anticancer activity in vitro and in the xenograft A2780/DDP tumor in a nude mouse model. This study suggests that chemotherapy in combination with TRAIL gene therapy may be an efficient approach to treat drug-resistant ovarian cancer.

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1.	SH LandisT MurrayS BoldenPA WingoCancer statistics, 1999CA Cancer J Clin49831199910.3322/canjclin.49.1.8
2.	SH KaufmannWC EarnshawInduction of apoptosis by cancer chemotherapyExp Cell Res2564249200110.1006/excr.2000.4838
3.	DE FisherApoptosis in cancer therapy: crossing the thresholdCell78539542199410.1016/0092-8674(94)90518-58069905
4.	CA SchmittSW LoweApoptosis is critical for drug response in vivoDrug Resist Updat4132134200110.1054/drup.2001.018811512522
5.	J BaselgaL NortonJ AlbanellYM KimJ MendelsohnRecombinant humanized anti-HER2 antibody (Herceptin) enhances the antitumor activity of paclitaxel and doxorubicin against HER2/neu overexpressing human breast cancer xenograftsCancer Res58282528311998
6.	M CuelloSA EttenbergMM NauS LipkowitzSynergistic induction of apoptosis by the combination of TRAIL and chemotherapy in chemoresistant ovarian cancer cellsGynecol Oncol81380390200110.1006/gyno.2001.619411371126
7.	CG FerreiraM EppingFA KruytG GiacconeApoptosis: target of cancer therapyClin Cancer Res820242034200212114400
8.	JP SheridanSA MarstersRM PittiControl of TRAIL-induced apoptosis by a family of signaling and decoy receptorsScience277818821199710.1126/science.277.5327.8189242611
9.	A AshkenaziTargeting death and decoy receptors of the tumour-necrosis factor superfamilyNat Rev Cancer2420430200210.1038/nrc82112189384
10.	H WalczakRE MillerK AriailTumoricidal activity of tumor necrosis factor related apoptosis-inducing ligand in vivoNat Med5157163199910.1038/55179930862
11.	MA GrotzerA EggertTJ ZuzakResistance to TRAIL-induced apoptosis in primitive neuroectodermal brain tumor cells correlates with a loss of caspase-8 expressionOncogene1946044610200010.1038/sj.onc.1203816
12.	H MaY LiuS LiuHF KungX SunD ZhengR XuRecombinant adeno-associated virus-mediated TRAIL gene therapy suppresses liver metastatic tumorInt J Cancer2314321200510.1002/ijc.2098215800912
13.	TS GriffithRD AndersonBL DavidsonRD WilliamsTL RatliffAdenoviral-mediated transfer of the TNF-related apoptosis-inducing ligand/apo-2 ligand gene induces tumor cell apoptosisJ Immunol16528862894200010.4049/jimmunol.165.5.288610946322
14.	JY SeolKH ParkCI HwangAdenovirus-TRAIL can overcome TRAIL resistance and induce a bystander effectCancer Gene Ther10540548200310.1038/sj.cgt.770059712833134
15.	Q PanB LiuJ LiuR CaiY WangC QianSynergistic induction of tumor cell death by combining cisplatin with an oncolytic adenovirus carrying TRAILMol Cell Biochem304315323200710.1007/s11010-007-9514-617577631
16.	MM KeaneSA EttenbergMM NauEK RussellS LipkowitzChemotherapy augments TRAIL-induced apoptosis in breast cell linesCancer Res5973474119999973225
17.	M NaganeG PanJJ WeddleVM DixitWK CaveneeHJ HuangIncreased death receptor 5 expression by chemotherapeutic agents in human gliomas causes synergistic cytotoxicity with tumor necrosis factor-related apoptosis-inducing ligand in vitro and in vivoCancer Res608478532000
18.	J KroslS BabanG KroslS RozenfeldC LargmanG SauvageauCellular proliferation and transformation induced by HOXB4 and HOXB3 proteins involves cooperation with PBX1Oncogene1634033412199810.1038/sj.onc.12018839692548
19.	QL JiangJM WangS JiangLM WenH ZhouLarge-scale real-time titration of green-fluorescence-protein-marked recombinant retrovirus: comparison with standard titration methodDi Yi Jun Yi Da Xue Xue Bao23110111032003(In Chinese)
20.	WS PearGP NolanML ScottD BaltimoreProduction of high-titer helper-free retroviruses by transient transfectionProc Natl Acad Sci USA9083928396199310.1073/pnas.90.18.83927690960
21.	R KassisF LarrousJ EstaquierH BourhyLyssavirus matrix protein induces apoptosis by a TRAIL-dependent mechanism involving caspase-8 activationJ Virol7865436555200410.1128/JVI.78.12.6543-6555.2004
22.	DW SeolJ LiMH SeolSY ParkRV TalanianTR BilliarSignaling events triggered by tumor necrosis factor-related apoptosis-inducing ligand (TRAIL): caspase-8 is required for TRAIL-induced apoptosisCancer Res61113811432001