RagD gene expression and NRF2 mutations in lung squamous cell carcinomas

  • Authors:
    • Hidefumi Sasaki
    • Masayuki Shitara
    • Keisuke Yokota
    • Yu Hikosaka
    • Satoru Moriyama
    • Motoki Yano
    • Yoshitaka Fujii
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  • Published online on: September 25, 2012     https://doi.org/10.3892/ol.2012.938
  • Pages: 1167-1170
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Abstract

RagD is a member of the small G protein family, which encodes a recently discovered activator of the mTOR pathway. In vitro, RagD plays an important role in the proliferation of NRF2 gene (NFE2L2) mutated cancer cells. We hypothesized that tumor RagD expression may be correlated with the mutation status of NRF2 in lung cancers. RagD mRNA levels were analyzed by quantitative real-time polymerase chain reaction (qPCR) in 90 surgically-treated lung squamous cell cancer cases, including 14 NRF2 mutation cases, and normalized by β-actin mRNA levels. Mean RagD/β‑actin mRNA levels of lung squamous cell carcinoma patients did not differ with age (≤65 vs. >65), Brinkman index (<400 vs. ≥400) or gender. RagD/β‑actin mRNA levels were significantly higher in stage III samples (3.204±3.623) compared to stage I samples (1.357±1.560) (P=0.0039). In addition, higher RagD/β-actin mRNA levels were identified in NRF2 mutant samples (3.107±3.633) compared to wild‑type samples (1.774±2.301) (P=0.074). These results suggest that RagD induction by NRF2 activation plays a role in the proliferation of lung squamous cell cancers.
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December 2012
Volume 4 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Sasaki H, Shitara M, Yokota K, Hikosaka Y, Moriyama S, Yano M and Fujii Y: RagD gene expression and NRF2 mutations in lung squamous cell carcinomas . Oncol Lett 4: 1167-1170, 2012.
APA
Sasaki, H., Shitara, M., Yokota, K., Hikosaka, Y., Moriyama, S., Yano, M., & Fujii, Y. (2012). RagD gene expression and NRF2 mutations in lung squamous cell carcinomas . Oncology Letters, 4, 1167-1170. https://doi.org/10.3892/ol.2012.938
MLA
Sasaki, H., Shitara, M., Yokota, K., Hikosaka, Y., Moriyama, S., Yano, M., Fujii, Y."RagD gene expression and NRF2 mutations in lung squamous cell carcinomas ". Oncology Letters 4.6 (2012): 1167-1170.
Chicago
Sasaki, H., Shitara, M., Yokota, K., Hikosaka, Y., Moriyama, S., Yano, M., Fujii, Y."RagD gene expression and NRF2 mutations in lung squamous cell carcinomas ". Oncology Letters 4, no. 6 (2012): 1167-1170. https://doi.org/10.3892/ol.2012.938