DNA hypomethylation of interleukin 8 in clear cell renal cell carcinoma

Yoo,Koo  Han; Park,Yong-Koo; Chang,Sung-Goo

doi:10.3892/ol.2012.992

DNA hypomethylation of interleukin 8 in clear cell renal cell carcinoma

Authors:
- Koo Han Yoo
- Yong-Koo Park
- Sung-Goo Chang
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Affiliations: Department of Urology, School of Medicine, Kyung Hee University, Seoul 130-702, Republic of Korea, Department of Pathology, School of Medicine, Kyung Hee University, Seoul 130-702, Republic of Korea
Published online on: October 24, 2012 https://doi.org/10.3892/ol.2012.992
Pages: 39-42

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Abstract

DNA hypomethylation is associated with carcinogenesis due to its involvement in cancer initiation and progression. In this study we analyzed the hypomethylation status of candidate genes in clear cell renal cell carcinoma (CCRCC) using a large-scale, high-throughput DNA methylation profiling technique. Results revealed that hypomethylated interleukin 8 (IL8) gene was the most prominent of the candidate genes, with a beta value difference of 0.406 (cancer tissue mean beta value, 0.346; normal tissue mean beta value, 0.752). Validation results using sequencing analysis demonstrated that the methylation rate was 2.4% in cancer tissue and 14.7% in normal tissue, whilst the non-methylation rate was 82.9% in cancer tissue and 52.9% in normal tissue. However, the hypomethylation status of IL8 had no significant relationship with Fuhrman's nuclear grade, tumor node metastasis (TNM) stage or survival (P>0.05). We revealed that the IL8 gene is maximally hypomethylated in cancer tissue compared to normal tissue.

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