The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue

  • Authors:
    • Peng Chen
    • Bing Liu
    • Ming Hu
  • View Affiliations

  • Published online on: January 7, 2013     https://doi.org/10.3892/ol.2013.1109
  • Pages: 947-952
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Abstract

The purpose of this study was to test hydroxycamptothecin (HCPT) and pingyangmycin (PYM) for their ability to inhibit the squamous cells of tongue carcinoma (Tca8113 cells). The effect of these compounds was tested using the MTT assay in vitro, clonogenic assays, flow cytometry, morphological observation, telomeric repeat amplification protocol (TRAP), transplantation of tumors into athymic mice and TUNEL staining. Treatment with HCPT and PYM, alone or in combination, inhibited the tumor cells and showed a greater inhibition when the drugs were combined. The cloning efficiency of Tca8113 cells was decreased. The microstructure and cell cycle of the cells changed significantly as a result of treatment. Telomerase activity was significantly inhibited in a time‑dependent manner. By appearing to promote apoptosis, the drugs demonstrated a significant level of inhibition of the tumor cells in an athymic mouse model, promoting prolonged survival. HCPT and PYM have a marked cytotoxic effect on Tca8113 cells which is improved when used in combination.
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March 2013
Volume 5 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen P, Liu B and Hu M: The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue. Oncol Lett 5: 947-952, 2013.
APA
Chen, P., Liu, B., & Hu, M. (2013). The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue. Oncology Letters, 5, 947-952. https://doi.org/10.3892/ol.2013.1109
MLA
Chen, P., Liu, B., Hu, M."The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue". Oncology Letters 5.3 (2013): 947-952.
Chicago
Chen, P., Liu, B., Hu, M."The effect of hydroxycamptothecin and pingyangmycin on human squamous cell carcinoma of the tongue". Oncology Letters 5, no. 3 (2013): 947-952. https://doi.org/10.3892/ol.2013.1109