Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells

Liang,Bo; Wang,Xin‑Jun; Shen,Pei‑Hong; Li,Xue‑Yuan; Cheng,Hong‑Wei; Shan,Qiao; Guo,Kui‑Yuan; Cao,Yu‑Wen; Fan,Qing‑Xia; Zheng,Rui‑Feng; Li,Bei; Zhang,Wei; Li,Yan‑Wei; Yang,Kai

doi:10.3892/ol.2013.1192

Synuclein‑γ suppression mediated by RNA interference inhibits the clonogenicity and invasiveness of MCF‑7 cells

Authors:
- Bo Liang
- Xin‑Jun Wang
- Pei‑Hong Shen
- Xue‑Yuan Li
- Hong‑Wei Cheng
- Qiao Shan
- Kui‑Yuan Guo
- Yu‑Wen Cao
- Qing‑Xia Fan
- Rui‑Feng Zheng
- Bei Li
- Wei Zhang
- Yan‑Wei Li
- Kai Yang
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Affiliations: Department of Neurosurgery, The Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Pathology, The Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Experimental Therapeutics, BC Cancer Agency Research Centre, Vancouver, British Columbia V5Z1L3, Canada, Department of General Surgery, The Fifth Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450052, P.R. China, Department of Oncology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou, Henan 450051, P.R. China, Center of Cancer Therapy, Henan People's Armed Police Corps Hospital, Zhengzhou, Henan 450052, P.R. China
Published online on: February 14, 2013 https://doi.org/10.3892/ol.2013.1192
Pages: 1347-1352

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Abstract

The aim of the present study was to investigate the effects of synuclein‑γ (SNCG) downregulation by RNA interference (RNAi) on the clonogenicity and invasiveness of MCF‑7 breast cancer cells. This study used four pairs of SNCG‑specific siRNAs which were designed and cloned into the pGPU6 plasmid for introduction into an MCF‑7 cell line. The SNCG knockdown efficacies of the four siRNAs were compared using the reverse transcription polymerase chain reaction (RT‑PCR) and immunocytochemistry. The cells' clonogenic and invasive phenotypes were examined with clonogenic and Boyden chamber assays. In comparison with the non‑specific siRNA and empty vector controls, all four SNCG siRNAs were observed to significantly inhibit SNCG expression at the mRNA and protein levels (F=481.06, P<0.001; F=147.42, P<0.0001). SNCG suppression mediated by RNAi successfully inhibited the clonogenicity (P=0.002) and invasiveness (P<0.001) of transfected MCF‑7 cells. According to the results of the present study, we concluded that SNCG suppression mediated by RNAi significantly suppressed SNCG expression at the mRNA and protein levels, suggesting that SNCG suppression mediated by an RNAi strategy may become a novel approach for treating advanced breast cancer.

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