Cytotoxic effect and tissue penetration of phenol for adjuvant treatment of giant cell tumours

Mittag,Falk; Leichtle,Carmen; Kieckbusch,Ina; Wolburg,Hartwig; Rudert,Maximilian; Kluba,Torsten; Leichtle,Ulf

doi:10.3892/ol.2013.1244

May 2013 Volume 5 Issue 5

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May 2013 Volume 5 Issue 5

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Article

Cytotoxic effect and tissue penetration of phenol for adjuvant treatment of giant cell tumours

Authors:
- Falk Mittag
- Carmen Leichtle
- Ina Kieckbusch
- Hartwig Wolburg
- Maximilian Rudert
- Torsten Kluba
- Ulf Leichtle
View Affiliations / Copyright

Affiliations: Department of Orthopaedic Surgery, University Hospital Tuebingen, Tuebingen 72076, Germany, Institute of Pathology and Neuropathology, Department of General Pathology, University Hospital Tuebingen, Tuebingen 72076, Germany, Department of Orthopaedic Surgery, University Hospital Wuerzburg, Wuerzburg 97074, Germany
Pages: 1595-1598
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Published online on: March 11, 2013

https://doi.org/10.3892/ol.2013.1244
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Abstract

Local adjuvant treatment of giant cell tumours (GCTs) of the bone with phenol has led to a significant reduction in recurrence rates. In the current study, the optimal phenol concentration and duration of intralesional exposure were evaluated. Specimens of GCTs were exposed to various concentrations of phenol solution (6, 60 and 80%) for either 1 or 3 min. Following embedding in glutaraldehyde, the tumour cell layers were examined by transmission electron microscopy. Destroyed cell organelles indicated the penetration depth as a sign of denaturation. Incubation of GCT specimens with 6% phenol solution for 3 min resulted in the most tissue damage and the deepest tissue penetration of ~200 µm. Incubation with 60 and 80% phenol solution reached a penetration depth of only ~100 µm. Phenol instillation may be used for the treatment of small scattered cellular debris following intralesional curettage; however, it is not suitable for treatment of remaining solid tumour tissue of GCT. The use of high phenol concentrations has no benefit and increases the risk of local or systemic intoxication.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Cytotoxic effect and tissue penetration of phenol for adjuvant treatment of giant cell tumours

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