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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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May 2013 Volume 5 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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Article

Ilex kudingcha C.J. Tseng (Kudingcha) has in vitro anticancer activities in MCF-7 human breast adenocarcinoma cells and exerts anti-metastatic effects in vivo

  • Authors:
    • Xin Zhao
    • Qiang Wang
    • Yu Qian
    • Jia-Le Song
  • View Affiliations / Copyright

    Affiliations: Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China, Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Republic of Korea
  • Pages: 1744-1748
    |
    Published online on: March 14, 2013
       https://doi.org/10.3892/ol.2013.1253
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Abstract

Ilex kudingcha C.J. Tseng (Kudingcha) is a traditional Chinese drink consumed in East Asia. The present study evaluated the in vitro anticancer effects of Kudingcha in MCF-7 human breast adenocarcinoma cells using a 3-(4,5-dimethyl‑2-thiazolyl)‑2,5-diphenyltetrazolium bromide (MTT) assay. At a concentration of 200 µg/ml, Kudingcha inhibited the growth of the MCF-7 cells by 81%. This was a greater degree of inhibition than that observed at concentrations of 100 and 50 µg/ml (58 and 19%, respectively). To elucidate the inhibitory mechanisms underlying the anticancer effect of Kudingcha in cancer cells, the expression of genes associated with apoptosis and inflammation were measured using RT-PCR. Kudingcha significantly induced apoptosis, as determined by 4,6-diamidino-2-phenylindole (DAPI) staining, by upregulating Bax, caspase-3 and caspase-9, and downregulating Bcl-2. The expression of the NF-κB, iNOS and COX-2 genes associated with inflammation was significantly decreased (P<0.05) by Kudingcha, thus demonstrating its anti-inflammatory properties. Kudingcha has been reported to exhibit inhibitory effects of tumor metastasis induced in 26-M3.1 colon carcinoma cells in BALB/c mice. The results demonstrated that Kudingcha had potent in vitro anticancer effects; it induced apoptosis, had anti-inflammatory activities and exerted in vivo anti-metastatic effects. Additionally, the anticancer, anti-inflammatory and anti-metastatic effects of Kudingcha were stronger at high concentrations than at low concentrations.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao X, Wang Q, Qian Y and Song J: Ilex kudingcha C.J. Tseng (Kudingcha) has in vitro anticancer activities in MCF-7 human breast adenocarcinoma cells and exerts anti-metastatic effects in vivo. Oncol Lett 5: 1744-1748, 2013.
APA
Zhao, X., Wang, Q., Qian, Y., & Song, J. (2013). Ilex kudingcha C.J. Tseng (Kudingcha) has in vitro anticancer activities in MCF-7 human breast adenocarcinoma cells and exerts anti-metastatic effects in vivo. Oncology Letters, 5, 1744-1748. https://doi.org/10.3892/ol.2013.1253
MLA
Zhao, X., Wang, Q., Qian, Y., Song, J."Ilex kudingcha C.J. Tseng (Kudingcha) has in vitro anticancer activities in MCF-7 human breast adenocarcinoma cells and exerts anti-metastatic effects in vivo". Oncology Letters 5.5 (2013): 1744-1748.
Chicago
Zhao, X., Wang, Q., Qian, Y., Song, J."Ilex kudingcha C.J. Tseng (Kudingcha) has in vitro anticancer activities in MCF-7 human breast adenocarcinoma cells and exerts anti-metastatic effects in vivo". Oncology Letters 5, no. 5 (2013): 1744-1748. https://doi.org/10.3892/ol.2013.1253
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao X, Wang Q, Qian Y and Song J: Ilex kudingcha C.J. Tseng (Kudingcha) has in vitro anticancer activities in MCF-7 human breast adenocarcinoma cells and exerts anti-metastatic effects in vivo. Oncol Lett 5: 1744-1748, 2013.
APA
Zhao, X., Wang, Q., Qian, Y., & Song, J. (2013). Ilex kudingcha C.J. Tseng (Kudingcha) has in vitro anticancer activities in MCF-7 human breast adenocarcinoma cells and exerts anti-metastatic effects in vivo. Oncology Letters, 5, 1744-1748. https://doi.org/10.3892/ol.2013.1253
MLA
Zhao, X., Wang, Q., Qian, Y., Song, J."Ilex kudingcha C.J. Tseng (Kudingcha) has in vitro anticancer activities in MCF-7 human breast adenocarcinoma cells and exerts anti-metastatic effects in vivo". Oncology Letters 5.5 (2013): 1744-1748.
Chicago
Zhao, X., Wang, Q., Qian, Y., Song, J."Ilex kudingcha C.J. Tseng (Kudingcha) has in vitro anticancer activities in MCF-7 human breast adenocarcinoma cells and exerts anti-metastatic effects in vivo". Oncology Letters 5, no. 5 (2013): 1744-1748. https://doi.org/10.3892/ol.2013.1253
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