Cyclooxygenase‑2 expression in the tumor environment is associated with poor prognosis in colorectal cancer patients

Lin,Peng‑Chan; Lin,Yih‑Jyh; Lee,Chung‑Ta; Liu,Hsiao‑Sheng; Lee,Jenq‑Chang

doi:10.3892/ol.2013.1426

Cyclooxygenase‑2 expression in the tumor environment is associated with poor prognosis in colorectal cancer patients

Authors:
- Peng‑Chan Lin
- Yih‑Jyh Lin
- Chung‑Ta Lee
- Hsiao‑Sheng Liu
- Jenq‑Chang Lee
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Affiliations: Department of Internal Medicine, College of Medicine, National Cheng Kung University, Tainan 704, Taiwan, R.O.C., Department of Surgery, College of Medicine, National Cheng Kung University, Tainan 704, Taiwan, R.O.C., Department of Pathology, College of Medicine, National Cheng Kung University, Tainan 704, Taiwan, R.O.C., Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan 704, Taiwan, R.O.C.
Published online on: June 26, 2013 https://doi.org/10.3892/ol.2013.1426
Pages: 733-739

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Abstract

The development of colorectal cancer (CRC) is commonly accompanied by the overexpression of the cyclooxygenase‑2 (COX‑2) gene, with high levels being most common in early colorectal lesions. In the present study, we hypothesized that the expression of COX‑2 in normal mucosa affects the expression of COX‑2 in adjacent tumors. COX‑2 protein expression levels were determined in tumor tissues and the adjacent normal mucosa of 49 paired clinical CRC specimens using western blotting and immunohistochemistry (IHC) staining. The majority of specimens exhibited an extremely low level of COX‑2 expression in the tumor tissue and a markedly higher expression level in the adjacent normal tissue, however, high COX‑2 expression in the tumor was shown to correlate with a high recurrence rate and poor overall survival. Of the nine CRC cell lines, HT29 showed consistently higher levels of COX‑2 expression. Therefore, COX‑2 expression in the normal tissue adjacent to the tumor may be involved in the tumorigenesis of CRC. These observations are likely to be useful in determining the significance of COX‑2 expression in the tumorigenesis of CRC.

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