A novel taspine derivative suppresses human liver tumor growth and invasion in vitro and in vivo

Wang,Nan; Zheng,Lei; Zhan,Yingzhuan; Zhang,Yanmin

doi:10.3892/ol.2013.1452

A novel taspine derivative suppresses human liver tumor growth and invasion in vitro and in vivo

Authors:
- Nan Wang
- Lei Zheng
- Yingzhuan Zhan
- Yanmin Zhang
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Affiliations: School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
Published online on: July 9, 2013 https://doi.org/10.3892/ol.2013.1452
Pages: 855-859

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Abstract

Taspine is an attractive target of research due to the anticancer and anti‑angiogenic effects shown by in vitro and in vivo experiments. The present study investigated the role of tas1611, which is a derivative of taspine that has increased activity and solubility, in the regulation of the invasive properties of the SMMC‑7721 liver cell line in vitro and in tumor inhibition in vivo. The proliferation of the SMMC‑7721 cells was examined using the tetrazole blue colorimetric method. Matrigel® invasion chamber assays and zymogram analyses were performed to assess the inhibitory effect of tas1611 on cell invasion. Finally, a solid tumor athymic mouse model was employed to further investigate the anti‑tumor effect of this compound. The results revealed that tas1611 had a marked inhibitory effect on the invasion of the SMMC‑7721 cells and that this effect was associated with the activity and expression levels of matrix metalloproteinase (MMP)‑2 and MMP‑9. Furthermore, tas1611 was able to inhibit tumor growth effectively in a solid tumor SMMC‑7721 athymic mouse model. In conclusion, tas1611 may serve as a promising novel therapeutic candidate for the treatment of metastatic liver cancer.

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