A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant

Cui,Liran; Sun,Yongxu; Xu,Hao; Xu,Huiyu; Cong,Huan; Liu,Jicheng

doi:10.3892/ol.2013.1484

A polysaccharide isolated from Agaricus blazei Murill (ABP‑AW1) as a potential Th1 immunity‑stimulating adjuvant

Authors:
- Liran Cui
- Yongxu Sun
- Hao Xu
- Huiyu Xu
- Huan Cong
- Jicheng Liu
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Affiliations: Department of Medicine Research, The First Affliated Hospital, Qiqihar Medical University, Qiqihar, Heilongjiang 161041, P.R. China, Department of Medicinal Chemistry and Biomacromolecules, Qiqihar Medical University, Qiqihar, Heilongjiang 161006, P.R. China
Published online on: July 23, 2013 https://doi.org/10.3892/ol.2013.1484
Pages: 1039-1044

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Abstract

In the present study, a low molecular weight polysaccharide, ABP‑AW1, isolated from Agaricus blazei Murill was assessed for its potential adjuvant activity. ABP‑AW1 is considered to create a ‘depot’ of antigen at a subcutaneous injection site. ICR mice were immunized with 100 µg ovalbumin (OVA) alone or with 100 µg OVA formulated in 0.9% saline containing 200 µg aluminum (alum) or ABP‑AW1 (50, 100 and 200 µg) on days 1 and 15. Two weeks after the secondary immunization, splenocyte proliferation, the expression of surface markers, cytokine production and the OVA‑specific antibody levels in the serum were determined. The OVA/ABP‑AW1 vaccine, in comparison with OVA alone, markedly increased the proliferation of splenic lymphocytes and elicited greater antigen‑specific CD4+ T cell activation, as determined by splenic CD4+CD69+ T cells and Th1 cytokine interferon (IFN)‑γ release. The combination of ABP‑AW1 and OVA also enhanced IgG2b antibody responses to OVA. In conclusion, these data indicated that ABP‑AW1 significantly enhanced the humoral and cellular immune responses against OVA in the mice, suggesting that ABP‑AW1 stimulated Th1‑type immunity. We suggest that ABP‑AW1 may serve as a new adjuvant.

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