Protective effect of tanshinone IIA against radiation‑induced ototoxicity in HEI-OC1 cells
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- Published online on: July 24, 2013 https://doi.org/10.3892/ol.2013.1486
- Pages: 901-906
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Abstract
Radiotherapy is a highly efficient treatment method for nasopharyngeal carcinoma that is often accompanied by significant ototoxic side‑effects. The inner ear hair cells are particularly prone to serious injury following radiotherapy. Tanshinone IIA is a transcription factor inhibitor that is extracted from the traditional herbal medicine, Salvia miltiorrhiza Bunge. The present study investigated the effects of tanshinone IIA treatment on radiation‑induced toxicity in the HEI‑OC1 hair cell line. Using an MTT assay and flow cytometry, the radiation‑induced weakening of the cells was observed to be alleviated when the cells were pre‑treated with tanshinone IIA. Radiation exposure promoted p65/nuclear factor (NF)‑κB nuclear translocation and activated the p53/p21 pathway, two processes which play a significant role in radiation‑induced cell apoptosis. However, pre‑treatment of the cells with tanshinone IIA inhibited p65/NF‑κB nuclear translocation and p53/p21 pathway activation. These results demonstrate that tanshinone IIA is capable of protecting cochlear cells from radiation‑induced injury through the suppression of p65/NF‑κB nuclear translocation and the p53/p21 signaling pathway.
