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U87MG glioma cells overexpressing IL‑17 acclerate early‑stage growth in vivo and cause a higher level of CD31 mRNA expression in tumor tissues

  • Authors:
    • Jinhui Hu
    • Hongxing Ye
    • Denghai Zhang
    • Weiwei Liu
    • Min Li
    • Ying Mao
    • Yuan Lu

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  • Published online on: August 7, 2013     https://doi.org/10.3892/ol.2013.1518
  • Pages: 993-999
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Abstract

Immunological alterations have been reported to be involved in glioma, the most common malignant disease of the adult brain. Our recent study identified higher levels of IL‑17 in glioma specimens. The present study investigated the role and possible mechanisms of IL‑17 in glioma tumorigenesis. Human IL‑17 cDNA was cloned and inserted into the eukaryotic pEGFP‑N1 expression vector, which was used to transfect the glioma U87MG cell line, resulting in a high level of IL‑17 expression in these cells. The cells were then transfected with IL‑17 (pEGFP‑N1‑IL‑17‑U87MG) or mock (pEGFP‑N1‑U87MG) vector or left untransfected (U87MG) and subcutaneously inoculated into the right flank of nude mice. The results revealed that the pEGFP‑N1‑IL‑17‑U87MG cells grew more rapidly in the early stages (P<0.05, determined on day 32 post‑inoculation compared with the other two groups). Quantitative (q)PCR detected higher mouse (m)CD31 mRNA levels in the IL‑17‑transfected group (P<0.01) compared with the mock‑transfected and untransfected groups. IL‑17 transfection altered the mRNA expression of a panel of molecules that are associated with immunity and inflammation in U87MG cells in vitro. An effect of the vector was identified, whereby the mock transfection strongly inhibited cell growth in vivo and dramatically altered the mRNA levels of multiple molecules in the cell culture in vitro compared with the untransfected cells. The present study confirmed that IL‑17 overexpression may enhance glioma cell growth in vivo, which may be associated with accelerated angiogenesis. IL‑17 overexpression may also alter the cellular mRNA expression of immune‑related molecules.
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Journal Cover

October 2013
Volume 6 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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  • Journal Metrics
  • Impact Factor: 2.5
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 5.7
  • CiteScore Rank: Q2: #139/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.574
  • SCImago Journal Rank (SJR): 0.644
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Spandidos Publications style
Hu J, Ye H, Zhang D, Liu W, Li M, Mao Y and Lu Y: U87MG glioma cells overexpressing IL‑17 acclerate early‑stage growth in vivo and cause a higher level of CD31 mRNA expression in tumor tissues. Oncol Lett 6: 993-999, 2013.
APA
Hu, J., Ye, H., Zhang, D., Liu, W., Li, M., Mao, Y., & Lu, Y. (2013). U87MG glioma cells overexpressing IL‑17 acclerate early‑stage growth in vivo and cause a higher level of CD31 mRNA expression in tumor tissues. Oncology Letters, 6, 993-999. https://doi.org/10.3892/ol.2013.1518
MLA
Hu, J., Ye, H., Zhang, D., Liu, W., Li, M., Mao, Y., Lu, Y."U87MG glioma cells overexpressing IL‑17 acclerate early‑stage growth in vivo and cause a higher level of CD31 mRNA expression in tumor tissues". Oncology Letters 6.4 (2013): 993-999.
Chicago
Hu, J., Ye, H., Zhang, D., Liu, W., Li, M., Mao, Y., Lu, Y."U87MG glioma cells overexpressing IL‑17 acclerate early‑stage growth in vivo and cause a higher level of CD31 mRNA expression in tumor tissues". Oncology Letters 6, no. 4 (2013): 993-999. https://doi.org/10.3892/ol.2013.1518