Identification of a cancer stem cell‑like side population in the HeLa human cervical carcinoma cell line

Wang,Kefang; Zeng,Jianfang; Luo,Lijing; Yang,Jiaxin; Chen,Jie; Li,Bin; Shen,Keng

doi:10.3892/ol.2013.1607

Identification of a cancer stem cell‑like side population in the HeLa human cervical carcinoma cell line

Authors:
- Kefang Wang
- Jianfang Zeng
- Lijing Luo
- Jiaxin Yang
- Jie Chen
- Bin Li
- Keng Shen
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Affiliations: Department of Obstetrics and Gynecology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China, Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China, Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, P.R. China
Published online on: October 7, 2013 https://doi.org/10.3892/ol.2013.1607
Pages: 1673-1680

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Abstract

The present study aimed to identify the stem cell characteristics of side population (SP) cells sorted from the widely‑used HeLa human cervical carcinoma cell line. The SP cells were sorted from the HeLa cell line using fluorescence‑activating cell sorting (FACS). Stem cell characteristics of the SP cells, including proliferation, self‑renewal, differentiation and the ability to form xenografts, were investigated in vitro and in vivo. The SP cells demonstrated strong tumorigenesis following in vivo transplantation into five to six‑week‑old female Balb/c mice. The SP cells were observed to be more resistant to chemotherapy and radiotherapy compared with non‑side population (NSP) cells. A higher expression of CD133 was observed in the SP cells compared with the NSP cells following FACS. The results demonstrated that the SP cells from the HeLa human cervical carcinoma cell line exhibit stem cell characteristics in vitro and also have a strong ability to form tumors in vivo. The cell surface marker CD133 may serve as a potential molecular marker for the identification of cervical cancer stem cells (CSCs).

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