Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
December 2013 Volume 6 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
December 2013 Volume 6 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2

  • Authors:
    • Bin Wei
    • Ying Song
    • Yonghong Zhang
    • Mingjun Hu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Xi'an First Hospital, Xi'an, Shaanxi 710002, P.R. China, Department of Gastroenterology, Central Hospital of Xi'an City, Xi'an, Shaanxi 710003, P.R. China
  • Pages: 1713-1718
    |
    Published online on: October 9, 2013
       https://doi.org/10.3892/ol.2013.1609
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:


Abstract

microRNAs (miRNAs or miRs) may function as oncogenes or tumor suppressors. The present study identified that miR‑449a was downregulated in human gastric cancer. The overexpression of miR‑449a inhibited gastric adenocarcinoma cell growth and promoted cell apoptosis in the MGC-803 and SGC-7901 gastric adenocarcinoma cell lines. Subsequently, Bcl‑2 was identified as a potential miR‑449a target by bioinformatics analysis. It was also shown that Bcl‑2 was negatively regulated by miR‑449a at the post‑transcriptional level, via a specific target site within the 3'-untranslated region (3'UTR), by luciferase reporter assay. The expression of miR‑449a induced cell apoptosis, as observed by TdT-mediated dUTP nick end labeling and caspase 3/7 assays, and was rescued by Bcl‑2 expression. Therefore, these observations indicate that miR‑449a acts as a tumor suppressor by targeting the Bcl‑2 gene and that it promotes gastric adenocarcinoma cell apoptosis via Bcl‑2. The findings of this study contribute to or current understanding of the functions of miR‑449a in gastric adenocarcinoma.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Yang L: Incidence and mortality of gastric cancer in China. World J Gastroenterol. 12:17–20. 2006.

2 

Ferlay J, Shin HR, Bray F, Forman D, Mathers C and Parkin DM: Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer. 127:2893–2917. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Saeki N, Ono H, Sakamoto H and Yoshida T: Genetic factors related to gastric cancer susceptibility identified using a genome-wide association study. Cancer Sci. 104:1–8. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Kim K, Chun KH, Suh PG and Kim IH: Alterations in cell proliferation related gene expressions in gastric cancer. Crit Rev Eukaryot Gene Expr. 21:237–254. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Lee S and Vasudevan S: Post-transcriptional stimulation of gene expression by microRNAs. Adv Exp Med Biol. 768:97–126. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Peralta-Zaragoza O, Bermudez-Morales VH and Madrid-Marina V: RNA interference: biogenesis molecular mechanisms and its applications in cervical cancer. Rev Invest Clin. 62:63–80. 2010.(In Spanish).

7 

Du L and Pertsemlidis A: microRNA regulation of cell viability and drug sensitivity in lung cancer. Expert Opin Biol Ther. 12:1221–1239. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Segura MF, Greenwald HS, Hanniford D, Osman I and Hernando E: MicroRNA and cutaneous melanoma: from discovery to prognosis and therapy. Carcinogenesis. 33:1823–1832. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Menendez P, Villarejo P, Padilla D, Menendez JM and Montes JA: Diagnostic and prognostic significance of serum microRNAs in colorectal cancer. J Surg Oncol. 107:217–220. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Liu H: MicroRNAs in breast cancer initiation and progression. Cell Mol Life Sci. 69:3587–3599. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Oulas A, Boutla A, Gkirtzou K, Reczko M, Kalantidis K and Poirazi P: Prediction of novel microRNA genes in cancer-associated genomic regions - a combined computational and experimental approach. Nucleic Acids Res. 37:3276–3287. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Peng S, Zeng X, Li X, Peng X and Chen L: Multi-class cancer classification through gene expression profiles: microRNA versus mRNA. J Genet Genomics. 36:409–416. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Thiel A and Ristimaki A: Gastric cancer: basic aspects. Helicobacter. 17(Suppl 1): 26–29. 2012. View Article : Google Scholar

14 

Sezer C, Yildirim M, Yildiz M, Sezgin A, Donem Dilli U, Goktas S and Bulbuller N: Prognostic significance of biological apoptosis factors in gastric cancer. J BUON. 18:138–146. 2013.PubMed/NCBI

15 

Amir S, Ma AH, Shi XB, Xue L, Kung HJ and Devere White RW: Oncomir miR-125b suppresses p14(ARF) to modulate p53-dependent and p53-independent apoptosis in prostate cancer. PLoS One. 8:e610642013. View Article : Google Scholar : PubMed/NCBI

16 

Chio CC, Lin JW, Cheng HA, Chiu WT, Wang YH, Wang JJ, Hsing CH and Chen RM: MicroRNA-210 targets antiapoptotic Bcl-2 expression and mediates hypoxia-induced apoptosis of neuroblastoma cells. Arch Toxicol. 87:459–468. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Zhang H, Li Y, Huang Q, Ren X, Hu H, Sheng H and Lai M: MiR-148a promotes apoptosis by targeting Bcl-2 in colorectal cancer. Cell Death Differ. 18:1702–1710. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Roth C, Pantel K, Muller V, Rack B, Kasimir-Bauer S, Janni W and Schwarzenbach H: Apoptosis-related deregulation of proteolytic activities and high serum levels of circulating nucleosomes and DNA in blood correlate with breast cancer progression. BMC Cancer. 11:42011. View Article : Google Scholar

19 

Wang PY, Li YJ, Zhang S, Li ZL, Yue Z, Xie N and Xie SY: Regulating A549 cells growth by ASO inhibiting miRNA expression. Mol Cell Biochem. 339:163–171. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Hoshyar R, Bathaie SZ and Sadeghizadeh M: Crocin triggers the apoptosis through increasing the Bax/Bcl-2 ratio and caspase activation in human gastric adenocarcinoma, AGS, cells. DNA Cell Biol. 32:50–57. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Hsu KW, Hsieh RH, Huang KH, Fen-Yau Li A, Chi CW, Wang TY, Tseng MJ, Wu KJ and Yeh TS: Activation of the Notch1/STAT3/Twist signaling axis promotes gastric cancer progression. Carcinogenesis. 33:1459–1467. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Yu JW, Wu JG, Zheng LH, Zhang B, Ni XC, Li XQ and Jiang BJ: Influencing factors and clinical significance of the metastatic lymph nodes ratio in gastric adenocarcinoma. J Exp Clin Cancer Res. 28:552009. View Article : Google Scholar : PubMed/NCBI

23 

Parkin DM, Bray F, Ferlay J and Pisani P: Global cancer statistics, 2002. CA Cancer J Clin. 55:74–108. 2005. View Article : Google Scholar

24 

Ali Z, Deng Y and Ma C: Progress of research in gastric cancer. J Nanosci Nanotechnol. 12:8241–8248. 2012. View Article : Google Scholar

25 

Liu XX, Li XJ, Zhang B, Liang YJ, Zhou CX, Cao DX, He M, Chen GQ, He JR and Zhao Q: MicroRNA-26b is underexpressed in human breast cancer and induces cell apoptosis by targeting SLC7A11. FEBS Lett. 585:1363–1367. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Skinnider BF, Horsman DE, Dupuis B and Gascoyne RD: Bcl-6 and Bcl-2 protein expression in diffuse large B-cell lymphoma and follicular lymphoma: correlation with 3q27 and 18q21 chromosomal abnormalities. Hum Pathol. 30:803–808. 1999. View Article : Google Scholar : PubMed/NCBI

27 

Korbakis D and Scorilas A: Quantitative expression analysis of the apoptosis-related genes BCL2, BAX and BCL2L12 in gastric adenocarcinoma cells following treatment with the anticancer drugs cisplatin, etoposide and taxol. Tumour Biol. 33:865–875. 2012. View Article : Google Scholar

28 

Zimmermann KC, Bonzon C and Green DR: The machinery of programmed cell death. Pharmacol Ther. 92:57–70. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Song KY, Jung CK, Park WS and Park CH: Expression of the antiapoptosis gene Survivin predicts poor prognosis of stage III gastric adenocarcinoma. Jpn J Clin Oncol. 39:290–296. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Boominathan L: The tumor suppressors p53, p63, and p73 are regulators of microRNA processing complex. PLoS One. 5:e106152010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wei B, Song Y, Zhang Y and Hu M: microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2. Oncol Lett 6: 1713-1718, 2013.
APA
Wei, B., Song, Y., Zhang, Y., & Hu, M. (2013). microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2. Oncology Letters, 6, 1713-1718. https://doi.org/10.3892/ol.2013.1609
MLA
Wei, B., Song, Y., Zhang, Y., Hu, M."microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2". Oncology Letters 6.6 (2013): 1713-1718.
Chicago
Wei, B., Song, Y., Zhang, Y., Hu, M."microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2". Oncology Letters 6, no. 6 (2013): 1713-1718. https://doi.org/10.3892/ol.2013.1609
Copy and paste a formatted citation
x
Spandidos Publications style
Wei B, Song Y, Zhang Y and Hu M: microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2. Oncol Lett 6: 1713-1718, 2013.
APA
Wei, B., Song, Y., Zhang, Y., & Hu, M. (2013). microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2. Oncology Letters, 6, 1713-1718. https://doi.org/10.3892/ol.2013.1609
MLA
Wei, B., Song, Y., Zhang, Y., Hu, M."microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2". Oncology Letters 6.6 (2013): 1713-1718.
Chicago
Wei, B., Song, Y., Zhang, Y., Hu, M."microRNA-449a functions as a tumor-suppressor in gastric adenocarcinoma by targeting Bcl‑2". Oncology Letters 6, no. 6 (2013): 1713-1718. https://doi.org/10.3892/ol.2013.1609
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team