MicroRNA‑145 inhibits the proliferation, migration and invasion of the human TCA8113 oral cancer line

Shao,Yuan; Zhang,Shao‑Qiang; Quan,Fang; Zhang,Peng‑Fei; Wu,Sheng‑Li

doi:10.3892/ol.2013.1621

MicroRNA‑145 inhibits the proliferation, migration and invasion of the human TCA8113 oral cancer line

Authors:
- Yuan Shao
- Shao‑Qiang Zhang
- Fang Quan
- Peng‑Fei Zhang
- Sheng‑Li Wu
View Affiliations

Affiliations: Department of Otorhinolaryngology, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, P.R. China, Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, P.R. China
Published online on: October 11, 2013 https://doi.org/10.3892/ol.2013.1621
Pages: 1636-1640

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The aim of this study was to investigate the effect of microRNA (miR)‑145 on the proliferation, migration and invasion of the human oral cancer line, TCA8113. Expression levels of miR‑145 in TCA8113 cells were detected by quantitative PCR. miR‑145 was transfected into human TCA8113 oral cancer cells and the proliferation, migration and invasion abilities of treated TCA8113 cells were detected by proliferation, migration and invasion assays, respectively. The expression levels of miR‑145 in TCA8113 cells were significantly lower than those in human normal oral keratinocytes (P<0.05). Cellular proliferation, migration and invasion abilities in the miR‑145 transfection group were significantly lower than those in the control group (all P<0.05). High miR‑145 expression was found to negatively regulate the proliferation, migration and invasion of TCA8113 cells. Results of the present study indicate that the expression of miR‑145 may be associated with the genesis and development of human oral cancer.

View Figures

View References

1	Warnalulasuriya S: Global epidemiology of oral and oropharyngeal cancer. Oral Oncol. 45:309–316. 2009. View Article : Google Scholar
2	Hernández-Guerrero JC, Jacinto-Alemán LF, Jiménez-Farfán MD, Macario-Hernández A, Hernández-Flores F and Alcántara-Vázquez A: Prevalence trends of oral squamous cell carcinoma. Mexico City’s General Hospital experience. Med Oral Patol Oral Cir Bucal. 18:e306–e311. 2013.PubMed/NCBI
3	Bagan J, Sarrion G and Jimenez Y: Oral cancer: clinical features. Oral Oncol. 46:414–417. 2010. View Article : Google Scholar
4	Shah JP and Gil Z: Current concepts in management of oral cancer-surgery. Oral Oncol. 45:394–401. 2009. View Article : Google Scholar : PubMed/NCBI
5	Kurita H, Nakanishi Y, Nishizawa R, Xiao T, Kamata T, et al: Impact of different surgical margin conditions on local recurrence of oral squamous cell carcinoma. Oral Oncol. 46:814–817. 2010. View Article : Google Scholar : PubMed/NCBI
6	Pérez I, Varona A, Blanco L, Gil J, Santaolalla F, et al: Increased APN/CD13 and acid aminopeptidase activities in head and neck squamous cell carcinoma. Head Neck. 31:1335–1340. 2009.PubMed/NCBI
7	Yi R and Fuchs E: MicroRNAs and their roles in mammalian stem cells. J Cell Sci. 124:1775–1783. 2011. View Article : Google Scholar : PubMed/NCBI
8	Valastyan S, Chang A, Benaich N, Reinhardt F and Weinberg RA: Activation of miR-31 function in already-established metastases elicits metastatic regression. Genes Dev. 25:646–659. 2011. View Article : Google Scholar
9	Hou J, Lin L, Zhou W, Wang Z, Ding G, et al: Identification of miRNomes in human liver and hepatocellular carcinoma reveals miR-199a/b-3p as therapeutic target for hepatocellular carcinoma. Cancer Cell. 19:232–243. 2011. View Article : Google Scholar : PubMed/NCBI
10	Hu J, Guo H, Li H, Liu Y, Liu J, et al: MiR-145 regulates epithelial to mesenchymal transition of breast cancer cells by targeting Oct4. PLoS One. 7:e459652012. View Article : Google Scholar : PubMed/NCBI
11	Hammond SM: MicroRNAs as tumor suppressors. Nat Genet. 39:582–583. 2007. View Article : Google Scholar : PubMed/NCBI
12	Sachdeva M and Mo YY: MicroRNA-145 suppresses cell invasion and metastasis by directly targeting mucin 1. Cancer Res. 70:378–387. 2010. View Article : Google Scholar : PubMed/NCBI
13	Cho WC, Chow AS and Au JS: Restoration of tumour suppressor hsa-miR-145 inhibits cancer cell growth in lung adenocarcinoma patients with epidermal growth factor receptor mutation. Eur J Cancer. 45:2197–2206. 2009. View Article : Google Scholar : PubMed/NCBI
14	Guo L, Liu Y, Bai Y, Sun Y, Xiao F and Guo Y: Gene expression profiling of drug-resistant small cell lung cancer cells by combining microRNA and cDNA expression analysis. Eur J Cancer. 46:1692–1702. 2010. View Article : Google Scholar : PubMed/NCBI
15	Yu Y, Chen W, Zhang Y, Hamburger AW, Pan H and Zhang Z: Suppression of salivary adenoid cystic carcinoma growth and metastasis by ErbB3 binding protein Ebp1 gene transfer. Int J Cancer. 120:1909–1913. 2007. View Article : Google Scholar : PubMed/NCBI
16	Cho WC: MicroRNAs: potential biomarkers for cancer diagnosis, prognosis and targets for therapy. Int J Biochem Cell Biol. 42:1273–1281. 2010. View Article : Google Scholar : PubMed/NCBI
17	Chiyomaru T, Enokida H, Tatarano S, Kawahara K, Uchida Y, et al: miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancer. Br J Cancer. 102:883–891. 2010. View Article : Google Scholar : PubMed/NCBI
18	Spizzo R, Nicoloso MS, Lupini L, Lu Y, Fogarty J, et al: miR-145 participates with Tp53 in a death-promoting regulatory loop and targets estrogen receptor-alpha in human breast cancer cells. Cell Death Differ. 17:246–254. 2010. View Article : Google Scholar : PubMed/NCBI
19	Gregersen LH, Jacobsen AB, Frankel LB, Wen J, Krogh A and Lund AH: MicroRNA-145 targets YES and STAT1 in colon cancer cells. PLoS One. 5:e88362010. View Article : Google Scholar : PubMed/NCBI
20	Kano M, Seki N, Kikkawa N, Fujimura L, Hoshino I, et al: MiR-145, miR-133a and miR-133b: Tumor suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma. Int J Cancer. 127:2804–2814. 2010. View Article : Google Scholar : PubMed/NCBI
21	Takagi T, Iio A, Nakagawa Y, Naoe T, Tanigawa N and Akao Y: Decreased expression of microRNA-143 and -145 in human gastric cancers. Oncology. 77:12–21. 2009. View Article : Google Scholar : PubMed/NCBI
22	Yu T, Wang XY, Gong RG, Li A, Yang S, et al: The expression profile of microRNAs in a model of 7,12-dimethyl-benz[a]anthrance-induced oral carcinogenesis in Syrian hamster. J Exp Clin Cancer Res. 28:642009.PubMed/NCBI
23	Sachdeva M, Zhu S, Wu F, Wu H, Walia V, et al: p53 represses c-Myc through induction of the tumor suppressor miR-145. Proc Natl Acad Sci USA. 106:3207–3212. 2009. View Article : Google Scholar : PubMed/NCBI
24	Chen X, Gong J, Zeng H, Chen N, Huang R, et al: MicroRNA-145 targets BNIP3 and suppresses prostate cancer progression. Cancer Res. 70:2728–2738. 2010. View Article : Google Scholar : PubMed/NCBI
25	Drebber U, Lay M, Wedemeyer I, Vallböhmer D, Bollschweiler E, et al: Altered levels of the onco-microRNA 21 and the tumor-supressor microRNAs 143 and 145 in advanced rectal cancer indicate successful neoadjuvant chemoradiotherapy. Int J Oncol. 39:409–415. 2011.
26	Chung YW, Bae HS, Song JY, Lee JK, Lee NW, et al: Detection of microRNA as novel biomarkers of epithelial ovarian cancer from the serum of ovarian cancer patient. Int J Gynecol Cancer. 23:673–679. 2013. View Article : Google Scholar : PubMed/NCBI
27	Shi B, Sepp-Lorenzino L, Prisco M, Linsley P, deAngelis T and Baserga R: Micro RNA-145 targets in insulin receptor substrate-1 and inhibits the growth of colon cancer cells. J Biol Chem. 282:32582–32590. 2007. View Article : Google Scholar : PubMed/NCBI
28	Zaman MS, Chen Y, Deng G, Shahryari V, Suh SO, et al: The functional significance of microRNA-145 in prostate cancer. Br J Cancer. 103:256–264. 2010. View Article : Google Scholar : PubMed/NCBI
29	Wang S, Bian C, Yang Z, Bo Y, Li J, et al: miR-145 inhibits breast cancer cell growth through RTKN. Int J Oncol. 34:1461–1466. 2009.PubMed/NCBI
30	Xu N, Papagiannakopoulos T, Pan G, Thomson JA and Kosik KS: MicroRNA-145 regulates OCT4, SOX2, and KLF4 and represses pluripotency in human embryonic stem cells. Cell. 137:647–658. 2009. View Article : Google Scholar : PubMed/NCBI