microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro

  • Authors:
    • Jianfeng Zhang
    • Xiaoling Kuai
    • Mengjiao Song
    • Xiaoqi  Chen
    • Zhihua Yu
    • Hong Zhang
    • Zhenbiao Mao
  • View Affiliations

  • Published online on: November 7, 2013     https://doi.org/10.3892/ol.2013.1667
  • Pages: 270-274
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Abstract

microRNAs (miRNAs) are a class of endogenously expressed, small non‑coding RNAs, which suppress their target mRNAs at the post‑transcriptional level. miRNAs play key roles in tumor metastasis. The aim of the present study was to investigate the expression of miRNA‑32 (miR‑32) on the biological behavior of the human gastric cancer cell line, SGC‑7901. SGC‑7901 cells were transfected with miR‑32‑mimic, miR‑32‑inhibitor and empty plasmid vectors using Lipofectamine™ 2000. The expression of GFP was observed by fluorescent microscopy and miR‑32 gene expression was detected by quantitative polymerase chain reaction. The cell counting kit‑8 assay was performed to evaluate the effect of miR‑32 expression on cell proliferation in vitro. Alterations in the migration and metastatic potential of SGC‑7901 cells, prior to and following miR‑32 gene transfection, were assayed by cell chemotactic migration and invasion tests. The results of the current study showed that the proliferation rate of the transfected SGC‑7901 cells overexpressing miR‑32 is reduced and cell chemotactic migration and invasion potentials is markedly reduced following miR‑32‑mimic transfection (P<0.05). In addition, the results demonstrated that overexpression of miR‑32 greatly inhibits the proliferation and decreases the migration and invasion capabilities of SGC‑7901 cells in vitro.
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2014-January
Volume 7 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang J, Kuai X, Song M, Chen X, Yu Z, Zhang H and Mao Z: microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro. Oncol Lett 7: 270-274, 2014
APA
Zhang, J., Kuai, X., Song, M., Chen, X., Yu, Z., Zhang, H., & Mao, Z. (2014). microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro. Oncology Letters, 7, 270-274. https://doi.org/10.3892/ol.2013.1667
MLA
Zhang, J., Kuai, X., Song, M., Chen, X., Yu, Z., Zhang, H., Mao, Z."microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro". Oncology Letters 7.1 (2014): 270-274.
Chicago
Zhang, J., Kuai, X., Song, M., Chen, X., Yu, Z., Zhang, H., Mao, Z."microRNA‑32 inhibits the proliferation and invasion of the SGC‑7901 gastric cancer cell line in vitro". Oncology Letters 7, no. 1 (2014): 270-274. https://doi.org/10.3892/ol.2013.1667