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2014-February Volume 7 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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Article

Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF‑7 cells

  • Authors:
    • Xiaoli Zhang
    • Zhifeng Wen
    • Xiaoyi Mi
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The First Affiliated Hospital and College of Basic Medical Sciences of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Neurosurgery, The First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 411-414
    |
    Published online on: November 25, 2013
       https://doi.org/10.3892/ol.2013.1703
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Abstract

Tumor necrosis factor (TNF) receptor‑associated factor 4 (TRAF4) was initially identified as a gene amplified and overexpressed in breast carcinoma. The present study investigated the expression and anti-apoptotic function of TRAF4 in human breast cancer MCF‑7 cells. TRAF4 was found to be localized in the cytoplasm and nuclei of MCF‑7 cells by immunofluorescence staining and western blotting. The expression of TRAF4 in normal MCF‑10A breast cells was found to be lower than in MCF‑7 and MDA‑MB‑231 breast cancer cells. Following TNF‑α treatment, TRAF4 depletion by siRNA in the MCF‑7 cells was observed to suppress cell proliferation and the nuclear expression of nuclear factor κB was significantly reduced. The percentage of early apoptotic cells in the MCF‑7 cells was augmented upon TRAF4‑knockdown, and an increase in G1 phase cells and a decrease in S phase cells was detected. These results indicate that TRAF4 has anti-apoptotic effects on apoptosis induced by TNF‑α in MCF‑7 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Wen Z and Mi X: Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF‑7 cells. Oncol Lett 7: 411-414, 2014.
APA
Zhang, X., Wen, Z., & Mi, X. (2014). Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF‑7 cells. Oncology Letters, 7, 411-414. https://doi.org/10.3892/ol.2013.1703
MLA
Zhang, X., Wen, Z., Mi, X."Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF‑7 cells". Oncology Letters 7.2 (2014): 411-414.
Chicago
Zhang, X., Wen, Z., Mi, X."Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF‑7 cells". Oncology Letters 7, no. 2 (2014): 411-414. https://doi.org/10.3892/ol.2013.1703
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Wen Z and Mi X: Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF‑7 cells. Oncol Lett 7: 411-414, 2014.
APA
Zhang, X., Wen, Z., & Mi, X. (2014). Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF‑7 cells. Oncology Letters, 7, 411-414. https://doi.org/10.3892/ol.2013.1703
MLA
Zhang, X., Wen, Z., Mi, X."Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF‑7 cells". Oncology Letters 7.2 (2014): 411-414.
Chicago
Zhang, X., Wen, Z., Mi, X."Expression and anti-apoptotic function of TRAF4 in human breast cancer MCF‑7 cells". Oncology Letters 7, no. 2 (2014): 411-414. https://doi.org/10.3892/ol.2013.1703
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