Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line

  • Authors:
    • Hui Zhang
    • Zeng‑Jun Guo
    • Wen‑Ming Xu
    • Xiao‑Juan You
    • Ling Han
    • Yan‑Xia Han
    • Liu‑Jiang Dai
  • View Affiliations

  • Published online on: December 6, 2013     https://doi.org/10.3892/ol.2013.1740
  • Pages: 525-530
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Abstract

In the present study, to identify the effective components of Chinese traditional herbs, Euphorbia hylonoma Hand.‑Mazz. (Euphorbiaceae), a folk herb that has been used among the Qinling mountain area for hundreds of years, was investigated. 3,3'‑Di‑O‑methyl ellagic acid‑4'‑O‑β‑d‑xylopyranoside (JNE2), an ellagic acid derivative, was isolated from the acetone extract of the herb and its antitumor activity against human hepatoma HepG2 cells was detected in vitro. The results showed that JNE2 inhibited the proliferation of HepG2 cells in a dose‑ and time‑dependent manner and blocked the cell cycle at the G1/S phase. A high dosage of JNE2 induced apoptosis of the tumor cells, but no significant differences were identified between the treatment groups. The invasiveness of HepG2 cells was also inhibited by JNE2. The mechanism of the antitumor effect of JNE2 at the molecular level was presumed to be due to the upregulation of the protein expression of Bax and caspase‑3, and the downregulation of the protein expression of Bcl‑2 and CCND1. The results suggested that JNE2 is a potential antitumor agent that merits further investigation.
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2014-February
Volume 7 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang H, Guo ZJ, Xu WM, You XJ, Han L, Han YX and Dai LJ: Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line. Oncol Lett 7: 525-530, 2014
APA
Zhang, H., Guo, Z., Xu, W., You, X., Han, L., Han, Y., & Dai, L. (2014). Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line. Oncology Letters, 7, 525-530. https://doi.org/10.3892/ol.2013.1740
MLA
Zhang, H., Guo, Z., Xu, W., You, X., Han, L., Han, Y., Dai, L."Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line". Oncology Letters 7.2 (2014): 525-530.
Chicago
Zhang, H., Guo, Z., Xu, W., You, X., Han, L., Han, Y., Dai, L."Antitumor effect and mechanism of an ellagic acid derivative on the HepG2 human hepatocellular carcinoma cell line". Oncology Letters 7, no. 2 (2014): 525-530. https://doi.org/10.3892/ol.2013.1740