Interferon‑α suppresses invasion and enhances cisplatin‑mediated apoptosis and autophagy in human osteosarcoma cells

  • Authors:
    • Jun Zhao
    • Mingli Wang
    • Zeng Li
    • Jingxian Chen
    • Zongshen Yin
    • Jun Chang
    • Dongmei Gao
    • Shiping Wang
  • View Affiliations

  • Published online on: December 16, 2013     https://doi.org/10.3892/ol.2013.1762
  • Pages: 827-833
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Abstract

Interferon (IFN)‑α is generated in response to viral infections and is used clinically in the therapy of a variety of viral infections and cancers. The present study investigated whether IFN‑α could inhibit the invasive ability of osteosarcoma cells using a Matrigel invasion assay. In addition, the osteosarcoma cells were treated with cisplatin and/or IFN‑α. Apoptosis and autophagy were assessed using 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay, Hoechst 33258 staining, flow cytometry assay, acridine orange staining, green fluorescent protein‑LC3 dot assay and transmission electron microscopy. Further analysis revealed that the efficacy of cisplatin was enhanced by the addition of the cytokine, IFN‑α. These results indicate that the combination therapy of chemotherapeutics and IFN‑α is a new approach for osteosarcoma, which requires validation by experiments in vivo.
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2014-March
Volume 7 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhao J, Wang M, Li Z, Chen J, Yin Z, Chang J, Gao D and Wang S: Interferon‑α suppresses invasion and enhances cisplatin‑mediated apoptosis and autophagy in human osteosarcoma cells. Oncol Lett 7: 827-833, 2014.
APA
Zhao, J., Wang, M., Li, Z., Chen, J., Yin, Z., Chang, J. ... Wang, S. (2014). Interferon‑α suppresses invasion and enhances cisplatin‑mediated apoptosis and autophagy in human osteosarcoma cells. Oncology Letters, 7, 827-833. https://doi.org/10.3892/ol.2013.1762
MLA
Zhao, J., Wang, M., Li, Z., Chen, J., Yin, Z., Chang, J., Gao, D., Wang, S."Interferon‑α suppresses invasion and enhances cisplatin‑mediated apoptosis and autophagy in human osteosarcoma cells". Oncology Letters 7.3 (2014): 827-833.
Chicago
Zhao, J., Wang, M., Li, Z., Chen, J., Yin, Z., Chang, J., Gao, D., Wang, S."Interferon‑α suppresses invasion and enhances cisplatin‑mediated apoptosis and autophagy in human osteosarcoma cells". Oncology Letters 7, no. 3 (2014): 827-833. https://doi.org/10.3892/ol.2013.1762