Significance of semaphorin‑3A and MMP‑14 protein expression in non‑small cell lung cancer

Zhou,Haiying; Wu,Aiping; Fu,Wei; Lv,Zheng; Zhang,Zhiyong

doi:10.3892/ol.2014.1920

Significance of semaphorin‑3A and MMP‑14 protein expression in non‑small cell lung cancer

Authors:
- Haiying Zhou
- Aiping Wu
- Wei Fu
- Zheng Lv
- Zhiyong Zhang
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Affiliations: Department of Thoracic Surgery, Tianjin Chest Hospital, Tianjin Medical University, Tianjin 300070, P.R. China, Department of Pathology, Hebei United University, Tangshan, Hebei 063000, P.R. China, Department of Thoracic Surgery, Tangshan Worker Hospital, Hebei Medical University, Tangshan, Hebei 063000, P.R. China, Department of Pathology, Tangshan Worker Hospital, Hebei Medical University, Tangshan, Hebei 063000, P.R. China
Published online on: February 27, 2014 https://doi.org/10.3892/ol.2014.1920
Pages: 1395-1400

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Abstract

Semaphorin‑3A is a chemorepellent guidance protein that is crucial in regulating the tumor microenvironment. MMP‑14, a membrane‑anchored matrix metalloproteinase, is closely associated with extracellular matrix (ECM) remodeling and cell migration in the progression of cancer metastasis. In the present study, the correlation between the expression levels of semaphorin‑3A and MMP‑14, and their subsequent prognostic significance in non‑small cell lung cancer (NSCLC), was investigated. The expression of semaphorin‑3A and MMP‑14 protein levels was analyzed in 94 cases of NSCLC tissues and in 80 cases of normal lung tissues, using immunohistochemistry (IHC). Correlation and survival analysis were used to further investigate their association and prognostic value. The results revealed that the NSCLC tissues exhibited a lower expression of semaphorin‑3A and a higher expression of MMP‑14 than in the control lung tissues. The downregulation of semaphorin‑3A and upregulation of MMP‑14 may promote pleural invasion, lymph node metastasis, vascular invasion and proliferating cell nuclear antigen expression. The expression of semaphorin‑3A was correlated with the maximum diameter of tumor. There was a negative correlation between the protein expression levels of semaphorin‑3A and MMP‑14 in NSCLC tissues. Furthermore, we identified that the patients with lower expression of semaphorin‑3A and a higher expression of MMP‑14 had worse disease prognosis. The data suggest that lower expression of semaphorin‑3A and a higher expression of MMP‑14 may promote occurrence and development in NSCLC and that the combined detection of semaphorin‑3A and MMP‑14 protein may be a helpful tool in predicting the prognosis of NSCLC.

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