Transactivation of proto‑oncogene c‑Myc by hepatitis B virus transactivator MHBst167

Lun,Yongzhi; Cheng,Jun; Chi,Qing; Wang,Xue‑Lei; Gao,Meng; Sun,Lida

doi:10.3892/ol.2014.2190

Transactivation of proto‑oncogene c‑Myc by hepatitis B virus transactivator MHBst167

Authors:
- Yongzhi Lun
- Jun Cheng
- Qing Chi
- Xue‑Lei Wang
- Meng Gao
- Lida Sun
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Affiliations: Liaoning Provincial University Key Laboratory of Biophysics, College of Medicine, Dalian University, Dalian, Liaoning 116622, P.R. China, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, P.R. China
Published online on: May 28, 2014 https://doi.org/10.3892/ol.2014.2190
Pages: 803-808

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Abstract

C‑terminally truncated hepatitis B virus (HBV) middle size surface proteins (MHBst) has been shown to be a transcriptional activator and may be relevant to hepatocarcinogenesis by transactivating gene expression. In the present study, a pcDNA3.1(‑)‑MHBst167 vector coding for MHBst truncated at amino acid 167 (MHBst167) was constructed and transfected into the HepG2 hepatoma cell line. mRNA and protein expression of MHBst167 in the cells was detected by reverse transcription‑polymerase chain reaction (RT‑PCR) and western blot analysis. A cDNA library of genes transactivated by the truncated protein in HepG2 cells was made in pGEM‑T Easy using suppression subtractive hybridization. The cDNAs were sequenced and analyzed with BLAST searching against the sequences in GenBank. The results showed that certain sequences, such as that of human proto‑oncogene c‑Myc, may be involved in tumor development. An expression vector pCAT3/c‑Myc containing the chloramphenicol acetyltransferase (CAT) gene under the control of a c‑Myc promoter was generated, and the transcriptional transactivating effect of MHBst167 on the c‑Myc promoter was investigated by RT‑PCR and western blotting. MHBst167 was found to upregulate the transcriptional activity of the promoter, as well as transcription and translation of c‑Myc. MHBst167 was also shown to transactivate SV40 immediate early promoter, and transcriptionally transactivate the expression of human c‑Myc. These findings provide new directions for studying the biological functions of MHBst167, and for a better understanding of the tumor development mechanisms of HBV infection.

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