Open Access

Small hairpin RNA-mediated Krüppel-like factor 8 gene knockdown inhibits invasion of nasopharyngeal carcinoma

  • Authors:
    • Jing Wang
    • Jian Li Hu
    • Ru Bo Cao
    • Qian Ding
    • Gang Peng
    • Shi Jiang Fei
    • Yao Jiang
    • Peng Cheng Li
    • Kun Yu Yang
    • Wen Jie Zhang
    • Gang Wu
    • Ruo Zheng Wang
    • Pin Dong Li
  • View Affiliations

  • Published online on: April 3, 2015     https://doi.org/10.3892/ol.2015.3099
  • Pages: 2515-2519
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to characterize the expression of Krüppel-like factor 8 (KLF8) in nasopahryngeal carcinoma (NPC) cell lines and determine its effect on tumor development and invasion following KLF8 gene knockdown by small hairpin RNA (shRNA). KLF8 expression in four NPC cell lines was examined by quantitative polymerase chain reaction (qPCR) and western blotting. KLF8 was knocked down in the SUNE1-5-8F/Sh-KLF8 cell line using shRNA, and the resulting stable cell line SUNE1‑5‑8F‑sh‑KLF8 was transplanted into nude mice in order to observe tumor formation and invasion. The results obtained from qPCR and western blotting revealed that, of the four NPC cell lines, KLF8 expression was lowest in the CNE‑1 cells and highest in the SUNE1‑5‑8F cells. The tumor xenograft mouse models revealed that SUNE1-5-8F/Sh-KLF8 cells had a reduced ability for tumor formation and invasion compared with the control group. These results demonstrated for the first time that KLF8 modulates the formation and invasive ability of nasopharyngeal carcinoma.
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June-2015
Volume 9 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang J, Hu JL, Cao RB, Ding Q, Peng G, Fei SJ, Jiang Y, Li PC, Yang KY, Zhang WJ, Zhang WJ, et al: Small hairpin RNA-mediated Krüppel-like factor 8 gene knockdown inhibits invasion of nasopharyngeal carcinoma. Oncol Lett 9: 2515-2519, 2015.
APA
Wang, J., Hu, J.L., Cao, R.B., Ding, Q., Peng, G., Fei, S.J. ... Li, P.D. (2015). Small hairpin RNA-mediated Krüppel-like factor 8 gene knockdown inhibits invasion of nasopharyngeal carcinoma. Oncology Letters, 9, 2515-2519. https://doi.org/10.3892/ol.2015.3099
MLA
Wang, J., Hu, J. L., Cao, R. B., Ding, Q., Peng, G., Fei, S. J., Jiang, Y., Li, P. C., Yang, K. Y., Zhang, W. J., Wu, G., Wang, R. Z., Li, P. D."Small hairpin RNA-mediated Krüppel-like factor 8 gene knockdown inhibits invasion of nasopharyngeal carcinoma". Oncology Letters 9.6 (2015): 2515-2519.
Chicago
Wang, J., Hu, J. L., Cao, R. B., Ding, Q., Peng, G., Fei, S. J., Jiang, Y., Li, P. C., Yang, K. Y., Zhang, W. J., Wu, G., Wang, R. Z., Li, P. D."Small hairpin RNA-mediated Krüppel-like factor 8 gene knockdown inhibits invasion of nasopharyngeal carcinoma". Oncology Letters 9, no. 6 (2015): 2515-2519. https://doi.org/10.3892/ol.2015.3099