JNK signaling pathway is involved in piperlongumine‑mediated apoptosis in human colorectal cancer HCT116 cells

Li,Wen; Wen,Chuangyu; Bai,Haiyan; Wang,Xiaoyan; Zhang,Xiaoli; Huang,Lanlan; Yang,Xiangling; Iwamoto,Aikichi; Liu,Huanliang

doi:10.3892/ol.2015.3371

JNK signaling pathway is involved in piperlongumine‑mediated apoptosis in human colorectal cancer HCT116 cells

Authors:
- Wen Li
- Chuangyu Wen
- Haiyan Bai
- Xiaoyan Wang
- Xiaoli Zhang
- Lanlan Huang
- Xiangling Yang
- Aikichi Iwamoto
- Huanliang Liu
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Affiliations: Guangdong Institute of Gastroenterology and The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, P.R. China, State Key Laboratory of Oncology in South China, Sun Yat‑sen University Cancer Center, Guangzhou, Guangdong 510080, P.R. China, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
Published online on: June 11, 2015 https://doi.org/10.3892/ol.2015.3371
Pages: 709-715
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Piperlongumine (PPLGM), an alkaloid isolated from the long pepper (Piper longum L.), can selectively trigger cancer cell death in colorectal cancer cells. The present study investigated whether the c‑Jun NH2‑terminal kinase (JNK) signaling pathway is involved in PPLGM‑induced apoptosis in the human colorectal cancer HCT116 cell line. The results demonstrated that PPLGM reduced the cell viability and induced cell apoptosis in a time‑ and concentration‑dependent manner, without a significant effect on cell cycle distribution. Meanwhile, treatment with 10 µM PPLGM resulted in JNK activation within 1 h, and a marked and sustained increase in c‑Jun phosphorylation in the HCT116 cells. In addition, SP600125, a general inhibitor of JNK, inhibited PPLGM‑induced apoptosis in the HCT116 cells by inhibiting PPLGM‑induced c‑Jun phosphorylation. Altogether, it can be concluded that the JNK signaling pathway, at least in part, is involved in PPLGM‑mediated apoptosis in HCT116 cells.

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