MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma

  • Authors:
    • Hui Li
    • Kun Zhang
    • Li‑Hong Liu
    • Yurong Ouyang
    • Hong‑Bin Guo
    • Hanchong Zhang
    • Jie Bu
    • Tao Xiao
  • View Affiliations

  • Published online on: June 16, 2015     https://doi.org/10.3892/ol.2015.3378
  • Pages: 1662-1668
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Abstract

MicroRNAs (miRNAs) are a family of small non‑protein coding RNAs, which regulate the expression of a wide variety of genes at the post‑transcriptional level to control numerous biological and pathological processes. Various circulating miRNAs have been identified as potential diagnostic and prognostic biomarkers in multiple types of cancer and disease. The aim of the present study was to identify potential miRNA biomarkers for the early diagnosis and relapse prediction of osteosarcoma (OS). miRNA profiling was performed on serum from patients with osteosarcoma and healthy controls. All putative miRNAs were verified by reverse transcription‑quantitative polymerase chain reaction analysis of 20 pre-therapeutic OS patients and 20 healthy individuals. The expression of miR‑106a‑5p, miR16‑5p, miR‑20a‑5p, miR‑425‑5p, miR451a, miR‑25‑3p and miR139‑5p was demonstrated to be downregulated in the serum of OS patients when compared with that of the healthy controls. Receiver‑operating characteristic curve analyses indicated that these 7 miRNAs may be used as diagnostic biomarkers with the ability to discriminate between the healthy cohort and patients with OS. These results provide novel insights into the use of miRNAs in early blood screening for OS.
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September-2015
Volume 10 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li H, Zhang K, Liu LH, Ouyang Y, Guo HB, Zhang H, Bu J and Xiao T: MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma. Oncol Lett 10: 1662-1668, 2015.
APA
Li, H., Zhang, K., Liu, L., Ouyang, Y., Guo, H., Zhang, H. ... Xiao, T. (2015). MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma. Oncology Letters, 10, 1662-1668. https://doi.org/10.3892/ol.2015.3378
MLA
Li, H., Zhang, K., Liu, L., Ouyang, Y., Guo, H., Zhang, H., Bu, J., Xiao, T."MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma". Oncology Letters 10.3 (2015): 1662-1668.
Chicago
Li, H., Zhang, K., Liu, L., Ouyang, Y., Guo, H., Zhang, H., Bu, J., Xiao, T."MicroRNA screening identifies circulating microRNAs as potential biomarkers for osteosarcoma". Oncology Letters 10, no. 3 (2015): 1662-1668. https://doi.org/10.3892/ol.2015.3378