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Reduced PTEN expression and overexpression of miR‑17‑5p, ‑19a‑3p, ‑19b‑3p, ‑21‑5p, ‑130b‑3p, ‑221‑3p and ‑222‑3p by glioblastoma stem‑like cells following irradiation

  • Authors:
    • Takatoshi Tokudome
    • Akiko Sasaki
    • Mayumi Tsuji
    • Yuko Udaka
    • Hideto Oyamada
    • Hiromichi Tsuchiya
    • Katsuji Oguchi

  • View Affiliations

  • Published online on: August 11, 2015     https://doi.org/10.3892/ol.2015.3594
  • Pages: 2269-2272
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Abstract

Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is a tumor suppressor gene that induces cell apoptosis by inhibiting the PI3K/Akt signaling pathway. Glioblastoma (GBM) is a brain tumor that is resistant to irradiation and chemotherapy and, thus, is difficult to cure. GBM stem‑like cells (GSCs) have been implicated as a cause of this resistance. microRNA (miRNA/miR) inhibits the expression of proteins. The objective of the present study was to identify miRNAs that target PTEN, which induces apoptosis, in irradiation‑resistant GSCs. When the expression of miRNAs was examined in GSCs irradiated at 60 Gy using the human GBM A172 cell line, the expression of PTEN‑targeting miR‑17‑5p, ‑19a‑3p, ‑19b‑3p, ‑21‑5p, ‑130b‑3p, ‑221‑3p and ‑222‑3p was significantly higher in irradiated GSCs than in non‑irradiated cells, and the PTEN expression levels, as revealed by immunostaining, were lower in the irradiated GSCs than in the non‑irradiated cells. These results suggested that the expression of PTEN was suppressed through the overexpression of PTEN‑targeting miRNAs in GSCs following irradiation.
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October-2015
Volume 10 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Tokudome T, Sasaki A, Tsuji M, Udaka Y, Oyamada H, Tsuchiya H and Oguchi K: Reduced PTEN expression and overexpression of miR‑17‑5p, ‑19a‑3p, ‑19b‑3p, ‑21‑5p, ‑130b‑3p, ‑221‑3p and ‑222‑3p by glioblastoma stem‑like cells following irradiation. Oncol Lett 10: 2269-2272, 2015.
APA
Tokudome, T., Sasaki, A., Tsuji, M., Udaka, Y., Oyamada, H., Tsuchiya, H., & Oguchi, K. (2015). Reduced PTEN expression and overexpression of miR‑17‑5p, ‑19a‑3p, ‑19b‑3p, ‑21‑5p, ‑130b‑3p, ‑221‑3p and ‑222‑3p by glioblastoma stem‑like cells following irradiation. Oncology Letters, 10, 2269-2272. https://doi.org/10.3892/ol.2015.3594
MLA
Tokudome, T., Sasaki, A., Tsuji, M., Udaka, Y., Oyamada, H., Tsuchiya, H., Oguchi, K."Reduced PTEN expression and overexpression of miR‑17‑5p, ‑19a‑3p, ‑19b‑3p, ‑21‑5p, ‑130b‑3p, ‑221‑3p and ‑222‑3p by glioblastoma stem‑like cells following irradiation". Oncology Letters 10.4 (2015): 2269-2272.
Chicago
Tokudome, T., Sasaki, A., Tsuji, M., Udaka, Y., Oyamada, H., Tsuchiya, H., Oguchi, K."Reduced PTEN expression and overexpression of miR‑17‑5p, ‑19a‑3p, ‑19b‑3p, ‑21‑5p, ‑130b‑3p, ‑221‑3p and ‑222‑3p by glioblastoma stem‑like cells following irradiation". Oncology Letters 10, no. 4 (2015): 2269-2272. https://doi.org/10.3892/ol.2015.3594